CatSper CRISPR/Cas9 KO Plasmid (h): sc-403796
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CatSper CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CatSper genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CatSper CRISPR/Cas9 KO Plasmid (h) | sc-403796 | 20 µg | $397.00 |
Overview
CATSPER1 encodes CatSper, a sperm-specific ion channel subunit required for calcium influx that drives hyperactivated motility and supports capacitation-associated signaling. CatSper channel activity integrates changes in membrane potential and intracellular Ca2+ dynamics to regulate flagellar beating patterns and downstream phosphorylation events essential for fertilization. Disruption of CATSPER1 is linked to impaired sperm function and is associated with male infertility phenotypes, making it a key target for mechanistic studies of sperm motility control. As a component of a multi-subunit channel complex, CatSper1 also serves as an entry point to investigate assembly, trafficking, and regulation of sperm ion channels.
CatSper CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CATSPER1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CATSPER1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CATSPER1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CatSper protein expression.
This CRISPR knockout system enables efficient generation of CATSPER1-deficient cell models for investigation of CatSper signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CATSPER1 exon(s) critical for CatSper function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CATSPER1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CatSper CRISPR/Cas9 KO Plasmid (h) and CatSper CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CATSPER1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CatSper HDR Plasmid (h) and CatSper HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CATSPER1 homology arms to support homology-directed repair at defined CATSPER1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.