CA XIV Double Nickase Plasmid (h): sc-404085-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CA XIV Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CA XIV Double Nickase Plasmid (h) and CA XIV Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CA14. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CA XIV Antibody (A-4): sc-166438
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CA XIV Double Nickase Plasmid (h) | sc-404085-NIC | 20 µg | $410.00 | |||
CA XIV Double Nickase Plasmid (h2) | sc-404085-NIC-2 | 20 µg | $410.00 |
Human CA14 encodes carbonic anhydrase XIV (CA XIV), a membrane-tethered zinc metalloenzyme that catalyzes the reversible hydration of CO₂ to bicarbonate and protons, supporting extracellular pH control and bicarbonate transport. By coupling with ion transporters and buffering systems, CA XIV contributes to acid–base homeostasis that shapes metabolic flux, epithelial physiology, and neuronal excitability. CA14 expression and CA XIV activity have been linked to processes that depend on precise pH regulation, including cell–cell signaling and microenvironmental acidification relevant to tumor biology and tissue remodeling. Dysregulated carbonic anhydrase activity is therefore studied in relation to altered pH dynamics observed across neurological and epithelial disorders and in cancers.
CA XIV Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CA14 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CA14. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CA14 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CA14-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.