Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
BVES CRISPR/Cas9 KO Plasmid (h): sc-403698
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- BVES CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the BVES genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: BVES Antibody (E-3): sc-374081
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
BVES CRISPR/Cas9 KO Plasmid (h) | sc-403698 | 20 µg | $397.00 |
Overview
Blood vessel epicardial substance (BVES), also known as POPDC1, is a membrane-associated protein enriched in striated muscle and epithelial tissues that contributes to cell–cell adhesion and epithelial polarity. BVES participates in signaling networks linked to cAMP binding, membrane trafficking, and cytoskeletal organization, helping coordinate junctional integrity and tissue architecture. Altered BVES expression or localization has been associated with disrupted barrier function and aberrant cell migration, making it relevant to studies of epithelial–mesenchymal transitions and tissue remodeling. In cancer biology and cardiovascular research, BVES is frequently examined for its roles in maintaining normal cellular organization and in pathways influencing proliferation and motility.
BVES CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the BVES gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the BVES together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the BVES open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish BVES protein expression.
This CRISPR knockout system enables efficient generation of BVES-deficient cell models for investigation of BVES signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting BVES exon(s) critical for BVES function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple BVES genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by BVES CRISPR/Cas9 KO Plasmid (h) and BVES CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the BVES locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by BVES HDR Plasmid (h) and BVES HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by BVES homology arms to support homology-directed repair at defined BVES target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.