BrdU Antibody (IIB5): sc-32323

BrdU Antibody (IIB5) is a mouse monoclonal IgG1 kappa light chain antibody that detects BrdU protein in non-species specific applications through immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-BrdU antibody (IIB5) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The halogenated pyrimidine analog bromodeoxyuridine (BrdU) plays a crucial role in DNA synthesis, incorporating into newly synthesized DNA strands during the S-phase of the cell cycle, making BrdU an invaluable tool for estimating the fraction of cells actively undergoing DNA replication. This incorporation allows researchers to quantify DNA synthesis and study cellular processes such as sister chromatid exchange and nascent DNA isolation. BrdU (IIB5) antibody binds specifically to exposed BrdU in single-stranded DNA following hydrochloric acid denaturation or nuclease digestion, which is essential for accurate detection. For optimal results, protease antigen recovery is recommended for tissues or cells fixed with crosslinking agents like formalin, although this may affect BrdU immunodetection specificity. BrdU (IIB5) monoclonal antibody shows cytoplasmic staining, providing clear visualization in immunohistochemistry and immunocytochemistry applications, thereby facilitating deeper understanding of cellular proliferation and DNA synthesis dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

BrdU Antibody (IIB5) References:

1. Pre-ribosomal RNA is processed in permeabilised cells at the site of transcription.  |  Stanek, D., et al. 2000. Eur J Cell Biol. 79: 202-7. PMID: 10777112
2. Exposure to continuous bromodeoxyuridine (BrdU) differentially affects cell cycle progression of human breast and bladder cancer cell lines.  |  Diermeier, S., et al. 2004. Cell Prolif. 37: 195-206. PMID: 15030553
3. Bromodeoxyuridine increases multipotency of human bone marrow-derived stem cells.  |  Qu, TY., et al. 2004. Restor Neurol Neurosci. 22: 459-68. PMID: 15798364
4. Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine.  |  Magaud, JP., et al. 1989. J Histochem Cytochem. 37: 1517-27. PMID: 2476478
5. Bromodeoxyuridine in tumors and chromosomes detected with a monoclonal antibody.  |  Morstyn, G., et al. 1983. J Clin Invest. 72: 1844-50. PMID: 6355188
6. The use of antibodies to 5-bromo-2'-deoxyuridine for the isolation of DNA sequences containing excision-repair sites.  |  Cohn, SM. and Lieberman, MW. 1984. J Biol Chem. 259: 12456-62. PMID: 6490625
7. Hydrochloric acid denaturation of colorectal tumour tissue infiltrated with bromodeoxyuridine.  |  Williamson, K., et al. 1994. Cytometry. 15: 162-8. PMID: 8168403
8. Protease antigen recovery decreases the specificity of bromodeoxyuridine detection in formalin-fixed tissue.  |  Bak, PM. and Panos, RJ. 1997. J Histochem Cytochem. 45: 1165-70. PMID: 9267477
9. Hyperthermia in the chick embryo: HSP and possible mechanisms of developmental defects.  |  Buckiová, D., et al. 1998. Int J Dev Biol. 42: 737-40. PMID: 9712529

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-BrdU Antibody (IIB5) has 253 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

BrdU Antibody (IIB5) Specifications

Alternate Product Name: Bromodeoxyuridine

Product Clone Name: IIB5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: Bromodeoxyuridine (BrdU)

Epitope Species: n/a

Product Reactivity/Detection: BrdU, a proliferation marker incorporated into newly synthesized DNA during S-phase of a cell cycle,

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 253 citations in various scientific publications

Available Conjugates/Forms for BrdU Antibody (IIB5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin