Molecular structure of β-Mercaptoethanol, CAS Number: 987-65-5
β-Mercaptoethanol (CAS 60-24-2)
See product citations (10)
Alternate Names:
BME
Application:
β-Mercaptoethanol is a reducing agent used in electrophoresis, amino acid detection, and distinguishing ssDNA/dsDNA)
CAS Number:
60-24-2
Purity:
≥99%
Molecular Weight:
78.10
Molecular Formula:
C2H6OS
Supplemental Information:
This is as a Dangerous Good for transport and may be subject to additional shipping charges.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
beta-Mercaptoethanol can act as an enzyme reactivator in systems necessitating reduction for activation, and has been used to reduce disulfide bonds in order to separate protein subunits for use in electrophoresis. This product can also be used to form o-Phthalaldehyde-beta-mercaptoethanol (OPAME), a fluorogenic reagent shown to detect amino acids based on its reaction with primary amines. More recently OPAME has been used to distinguish between ssDNA/dsDNA and even single base mismatches in the hybridization of DNA.
β-Mercaptoethanol (CAS 60-24-2) References
- The activation mechanism of human porphobilinogen synthase by 2-mercaptoethanol: intrasubunit transfer of a reserve zinc ion and coordination with three cysteines in the active center. | Sawada, N., et al. 2005. J Biol Inorg Chem. 10: 199-207. PMID: 15747133
- Conformational change detection of DNA with the fluorogenic reagent of o-phthalaldehyde-beta-mercaptoethanol. | Long, YF., et al. 2008. J Phys Chem B. 112: 1783-8. PMID: 18215031
- Effect of thiol compounds on bleomycin-induced DNA and chromosome damage in human cells. | Mira, A., et al. 2013. Arch Environ Occup Health. 68: 107-16. PMID: 23428061
- Detection of plant oil DNA using high resolution melting (HRM) post PCR analysis: a tool for disclosure of olive oil adulteration. | Vietina, M., et al. 2013. Food Chem. 141: 3820-6. PMID: 23993554
- Cryo-EM Structures Reveal Mechanism and Inhibition of DNA Targeting by a CRISPR-Cas Surveillance Complex. | Guo, TW., et al. 2017. Cell. 171: 414-426.e12. PMID: 28985564
- Coordinated Control of mRNA and rRNA Processing Controls Embryonic Stem Cell Pluripotency and Differentiation. | Corsini, NS., et al. 2018. Cell Stem Cell. 22: 543-558.e12. PMID: 29625069
- A coculture system composed of THP-1 cells and 3D reconstructed human epidermis to assess activation of dendritic cells by sensitizing chemicals after topical exposure. | Schellenberger, MT., et al. 2019. Toxicol In Vitro. 57: 62-66. PMID: 30771472
- Stimulation of Hair Growth by Small Molecules that Activate Autophagy. | Chai, M., et al. 2019. Cell Rep. 27: 3413-3421.e3. PMID: 31216464
- Histone Modifications Regulate Chromatin Compartmentalization by Contributing to a Phase Separation Mechanism. | Wang, L., et al. 2019. Mol Cell. 76: 646-659.e6. PMID: 31543422
- SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer. | Lopitz-Otsoa, F., et al. 2019. J Vis Exp.. PMID: 31736480
- N-glycan in the scavenger receptor cysteine-rich domain of hepsin promotes intracellular trafficking and cell surface expression. | Sun, S., et al. 2020. Int J Biol Macromol. 161: 818-827. PMID: 32553951
- Systematic Activity Maturation of a Single-Domain Antibody with Non-canonical Amino Acids through Chemical Mutagenesis. | Lindstedt, PR., et al. 2021. Cell Chem Biol. 28: 70-77.e5. PMID: 33217338
- Condensed Chromatin Behaves like a Solid on the Mesoscale In Vitro and in Living Cells. | Strickfaden, H., et al. 2020. Cell. 183: 1772-1784.e13. PMID: 33326747
- SARS-CoV-2 Nsp14 activates NF-κB signaling and induces IL-8 upregulation. | Li, T., et al. 2021. bioRxiv.. PMID: 34075374
- Flow Cytometry-Based Determination of Ploidy from Dried Leaf Specimens in Genomically Complex Collections of the Tropical Forage Grass Urochloa s. l. | Tomaszewska, P., et al. 2021. Genes (Basel). 12: PMID: 34201593
- Jack bean urease (EC 3.5.1.5). III. The involvement of active-site nickel ion in inhibition by beta-mercaptoethanol, phosphoramidate, and fluoride. | Dixon, NE., et al. 1980. Can J Biochem. 58: 481-8. PMID: 7272836
Inhibitor of:
α-S1-casein, ACYP2, Anamorsin, Arylsulfatase K, BFP, CHIC2, Cytokeratin 2E, GCL, Hen Egg Lysozyme, ISCA1/1L, KRTAP13-1, KRTAP13-2, KRTAP19-4, KRTAP2, Krtap28-10, KRTAP4-13, KRTAP4-2, KRTAP4-4, KRTAP9-4, KRTAP9-8, MSP-1, MUP6, Obp1b, OR1A1, peroxidase, Syntaxin 16, TXNDC16, ZNF202, and ZNF256.Activator of:
3010026O09Rik, 3110003A17Rik, 4732456N10Rik, 9030025P20Rik, Abin-3, AGR2, Aldolase, ALR, ATF-6β, ATPBD3, BC003883, Bpifb9b, BPV E2, BRINP2, C4orf52, Cardiotrophin-2, caspase-12, CD300E, CDO1, CHP1, CKMT1B, COX4NB, CRELD2, DAD1, Dematin, DNASE2B, DPY19L3, EDEM3, EF-Ts, EG433365, eIF2Bδ, ERGIC-53, Ero1-Lα, ERp19, ERp46, ERp57, FEM-2, FN3KRP, Fus3, Gm97, GPT, GRP 94, H2-T3, IgG Chain C, Isthmin, ITM1, Keratin 74, Kex2, LSm1, MAP7D3, MCT14, MHC class II I E, NACAD, Neurogenin 3, NFAM1, NFU1, Oct-3A, Oct-4, oogenesin 2, oogenesin 3, oogenesin 4, OS-9, PAPD5, PDI1 ER and Nuclear Envelope Marker, PDIA2, PDILT, PDIR, PGCP, Ppat, Prdx6b, Pronase, quiescin Q6, R-PE, Rer1, Rhodanese, RISC, RNase 1, RNase 4, RNase K, Rslcan-8, Sel-1L2, selenocysteine lyase, Selenoprotein O, Sm B, SPC12, Stat5b, T1-cadherin, TMEM111, TRA-1-60, TRAP-δ, TRITC, Trx-2, TXNDC14, TXNDC16, UBXD5, VKOR, WTIP, and ZDHHC18.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
β-Mercaptoethanol, 100 ml | sc-202966A | 100 ml | $90.00 | |||
β-Mercaptoethanol, 250 ml | sc-202966 | 250 ml | $120.00 |