Anticorpo Arginase 2/ARG2 (A-10) raccomandato per il rilevamento di Arg2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA

Anticorpo Arginase 2/ARG2 (A-10): sc-393496

Anticorpo Arginase 2/ARG2 (A-10) valutazione 4.8 di 5 di 5.
  • y_2021, m_6, d_20, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_it_IT, sid_393496, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 106ms
  • REVIEWS, PRODUCT
loading
5
4
4
1
3
0
2
0
1
0

Schede tecniche

    • Anti-Arginase 2/ARG2 Antibody A-10 è un monoclonale di topo IgA κ Arginase 2/ARG2 antibody, citato in 5 pubblicazioni, fornito in 200 µg/ml
    • specifico per un epitopo mappato tra gli amminoacidi 304-335 vicino al C-terminus di arginase II di origine human
    • raccomandato per il rilevamento di Arg2 di origine mouse, rat e human in WB, IP, IF, IHC(P) e ELISA; anche reattivo con ulteriori specie, incluse e canine, bovine and porcine
    • disponibile coniugato ad agarosio per IP; e a phycoerythrin, FITC, Alexa Fluor® 488 o Alexa Fluor® 647 per IF, IHC(P) e FCM
    • Contattaci per ricevere un FREE 10 µg sample di Arginase 2/ARG2 (A-10): sc-393496.
loading

Why does the image in the datasheet show a band size of 90kDa when the expected protein size is 40kDa. This antibody is suggested as a replacement for H-64 which was a rabbit monoclonal at 40kDa. So does this not detect the same protein? Thanks.

Richiesta da: stonology
Thank you for your question. According to the literature and our data in arginase II transfected lysates, the expected molecular weight for arginase II is approximately 40 kda. Unfortunately, we do not have any reference to support a molecular weight of 90 kda in cell lines at this time. Therefore, we are going to remove that data from our website and reevaluate the antibody in different cell lines. This mouse monoclonal antibody, arginase II (A-10): sc-393496, does detect the same arginase II protein as the now discontinued rabbit polyclonal antibody.
Risposta da: Technical Support
Data di pubblicazione: 2017-12-07

I have two questions. First, in your data sheet, molecular weights is 40kDa. But figure of WB band says 90kDa. Is it fusion band? I wonder real band size.  Next, what secondary antibody should I use for western blotting? 

Richiesta da: Image
Thank you for your question. According to the literature and our data in arginase II transfected lysates, the expected molecular weight for arginase II is approximately 40 kda. Unfortunately, we do not have any reference to support a molecular weight of 90 kda in cell lines at this time. Therefore, we are going to remove that data from our website and reevaluate the antibody in different cell lines. This mouse monoclonal antibody, arginase II (A-10): sc-393496, is a mouse monoclonal IgA (kappa light chain). Ideally, we would recommend using an isotype-specific secondary antibody (anti-mouse IgA) for detection. Unfortunately, we do not currently offer this type of secondary antibody for sale.
Risposta da: Technical Support
Data di pubblicazione: 2017-11-22

What blocking solution should I use with arginase II (A-10): sc-393496 monoclonal antibody for Western blot?

Richiesta da: jenni
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Risposta da: Technical Support 5
Data di pubblicazione: 2017-02-09
  • y_2021, m_6, d_20, h_18CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_it_IT, sid_393496, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 135ms
  • QUESTIONS, PRODUCT
Rated 4 di 5 di da Good result I got free 10 µg sample of arginase II (A-10): sc-393496 one week ago, and prepared the HPF cell sample immidely, perfect WB result was obtained yesterday, the dilution of this antibody was 1:500, and incubated overnight at 4.
Data di pubblicazione: 2017-09-22
Rated 5 di 5 di da Good performance, which really help me quite a lot two weeks ago , I applied the free sample for pre-experiment, THe results turns out to be satisfactory. I really appreciate that. and the delivery service is good, thanks
Data di pubblicazione: 2017-08-17
Rated 5 di 5 di da A good choice. This antibody has some great data to support it. Immunostaining and IF is bright, clear and specific. The WB data shows strong and specific bands. This antibody is reactive with a broad range of species.
Data di pubblicazione: 2017-01-25
Rated 5 di 5 di da Good signal strength in human arginase II Good signal strength in human arginase II transfected 293T whole cell lysate. -SCBT QC
Data di pubblicazione: 2015-04-07
Rated 5 di 5 di da Works well for IF applications Works well for IF applications, positive in HeLa. -SCBT QC
Data di pubblicazione: 2015-01-02
  • y_2021, m_6, d_20, h_18
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.17
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_it_IT, sid_393496, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 14ms
  • REVIEWS, PRODUCT