Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Aldolase B Double Nickase Plasmid (h): sc-402714-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Aldolase B Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Aldolase B Double Nickase Plasmid (h) and Aldolase B Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ALDOB. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Aldolase B Antibody (C-11): sc-393278
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Aldolase B Double Nickase Plasmid (h) | sc-402714-NIC | 20 µg | $410.00 | |||
Aldolase B Double Nickase Plasmid (h2) | sc-402714-NIC-2 | 20 µg | $410.00 |
ALDOB encodes aldolase B, a fructose-bisphosphate aldolase that catalyzes key steps in fructose metabolism and glycolysis/gluconeogenesis by cleaving fructose-1-phosphate and fructose-1,6-bisphosphate into triose phosphates. In human liver, kidney, and intestinal epithelium, aldolase B supports carbon flux into energy production and biosynthetic pathways, linking dietary fructose handling to central carbohydrate homeostasis. Altered ALDOB expression or activity perturbs fructose catabolism and downstream metabolic signaling, and is associated with inborn errors of metabolism such as hereditary fructose intolerance as well as broader metabolic dysregulation contexts studied in hepatocyte biology. Consequently, ALDOB is frequently used as a functional marker in hepatic differentiation and as a node for investigating nutrient-responsive transcriptional programs.
Aldolase B Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ALDOB locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ALDOB. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ALDOB function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ALDOB-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.