5730419I09Rik Activators
Chemical activators of C2 calcium-dependent domain containing 5 can initiate its activation through a variety of mechanisms, primarily involving the regulation of intracellular calcium concentration and protein phosphorylation. Calcium chloride, for example, provides a direct source of calcium ions, which are essential for the activation of C2 calcium-dependent domain containing 5. These ions bind to the protein's C2 domain, triggering a conformational change that is critical for its functional engagement with cellular membranes and other proteins. Similarly, ionomycin and A23187, both calcium ionophores, elevate the intracellular calcium levels, facilitating the binding of calcium to the C2 domain of C2 calcium-dependent domain containing 5, inducing its activation. Thapsigargin, by inhibiting the SERCA, causes a rise in cytosolic calcium concentration that, in turn, leads to activation of C2 calcium-dependent domain containing 5 via increased calcium binding to its C2 domain. Ionophore X-537A also raises intracellular calcium, endorsing the activation of the protein by the same mechanism. Furthermore, BAPTA-AM, after conversion in the cell, liberates calcium ions that can bind to and activate C2 calcium-dependent domain containing 5.
In addition to calcium-mediated activation, C2 calcium-dependent domain containing 5 can be activated through phosphorylation by protein kinase C (PKC). Phorbol 12-myristate 13-acetate (PMA), bryostatin 1, OAG, 4β-phorbol, and TPA are all activators of PKC, which in turn phosphorylates C2 calcium-dependent domain containing 5, enhancing its ability to interact with calcium and participate in cellular signaling. This phosphorylation by PKC effectively amplifies the protein's ability to associate with cellular membranes and to engage in its signaling functions. Dibucaine, although not directly related to PKC activation, increases intracellular calcium concentration, thus contributing to the activation of C2 calcium-dependent domain containing 5 through enhanced calcium binding to its C2 domain. Collectively, these chemicals can activate C2 calcium-dependent domain containing 5 by modifying intracellular calcium dynamics or by inducing post-translational modifications such as phosphorylation.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
Calcium chloride anhydrous | 10043-52-4 | sc-207392 sc-207392A | 100 g 500 g | $66.00 $262.00 | 1 | |
Calcium chloride can directly activate C2 calcium-dependent domain containing 5 protein by providing the essential calcium ions necessary for its calcium-dependent activation. This binding of calcium ions to the C2 domain induces a conformational change that enables the protein to interact with phospholipid membranes or other proteins, initiating its function. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which in turn can activate C2 calcium-dependent domain containing 5 by phosphorylation, a modification that often regulates protein function. PKC-mediated phosphorylation can enhance the protein's ability to bind calcium and interact with its targets, leading to its functional activation. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that increases intracellular calcium levels, which can activate C2 calcium-dependent domain containing 5 by facilitating the binding of calcium to its C2 domain. This calcium binding is crucial for the activation and function of the protein in cellular signaling pathways. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin inhibits the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), leading to an increase in cytosolic calcium levels. Elevated calcium can activate C2 calcium-dependent domain containing 5 by promoting calcium binding to its C2 domain, which is required for its activation and subsequent biological activity. | ||||||
Bryostatin 1 | 83314-01-6 | sc-201407 | 10 µg | $245.00 | 9 | |
Bryostatin 1 is a modulator of PKC, which can lead to the activation of C2 calcium-dependent domain containing 5 by PKC-mediated phosphorylation. This phosphorylation can enhance the protein's calcium-dependent functions and interactions with other molecules, resulting in its activation. | ||||||
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
A23187 acts as a calcium ionophore, augmenting intracellular calcium levels and thereby can activate C2 calcium-dependent domain containing 5. The increased calcium concentration in the cell facilitates the binding of calcium to the protein's C2 domain, which is essential for its activation. | ||||||
BAPTA/AM | 126150-97-8 | sc-202488 sc-202488A | 25 mg 100 mg | $138.00 $458.00 | 61 | |
BAPTA-AM is a cell-permeable calcium chelator that, after hydrolysis in the cell, increases the availability of free calcium ions. These free calcium ions can activate C2 calcium-dependent domain containing 5 by binding to its C2 domain, thereby inducing conformational changes that activate the protein. | ||||||
1-Oleoyl-2-acetyl-sn-glycerol (OAG) | 86390-77-4 | sc-200417 sc-200417A | 10 mg 50 mg | $119.00 $453.00 | 1 | |
OAG is an analog of diacylglycerol (DAG) and can activate PKC, subsequently leading to the activation of C2 calcium-dependent domain containing 5 by PKC-mediated phosphorylation. This phosphorylation can modulate the protein's interaction with calcium and its membrane association, thus activating it. | ||||||
Phorbol | 17673-25-5 | sc-253267 | 5 mg | $270.00 | 1 | |
4β-Phorbol is an activator of PKC, which can lead to subsequent phosphorylation and activation of C2 calcium-dependent domain containing 5. By stimulating PKC, which then targets the protein for phosphorylation, it can enhance the protein's activity and its role in signaling pathways. | ||||||