4922502D21Rik Activators
Chemical activators of Calcium Ionophore A23187, Zinc Chloride, Manganese(II) Chloride, Cobalt(II) Chloride, Thapsigargin, Phorbol 12-Myristate 13-Acetate (PMA), Ionomycin, N-Formylmethionyl-leucyl-phenylalanine (FMLP), Forskolin, Dibutyryl-cAMP, Sodium Orthovanadate, and Brefeldin A can all play roles in the activation of the protein CLEC2M. These chemicals engage with intracellular pathways and structural components of the protein to modulate its activity. For instance, Calcium Ionophore A23187 and Ionomycin, both calcium ionophores, directly increase the intracellular concentration of calcium, a crucial cofactor for the activation of CLEC2M, facilitating its binding to glycoprotein ligands. Thapsigargin also raises cytosolic calcium levels by inhibiting the endoplasmic reticulum Ca2+ ATPase, thereby contributing to CLEC2M activation.
On the other hand, Zinc Chloride and Cobalt(II) Chloride provide essential metal ions that maintain the structural integrity and conformation of CLEC2M, ensuring it is primed for ligand interaction. Manganese(II) Chloride supplies manganese ions, which can activate kinases that phosphorylate proteins, potentially including CLEC2M, leading to its activation through post-translational modifications. Similarly, Phorbol 12-Myristate 13-Acetate (PMA) activates protein kinase C (PKC), which can phosphorylate CLEC2M, thereby enhancing its activity. FMLP, acting through G-protein-coupled receptors, can result in increased intracellular calcium, which subsequently activates CLEC2M. Forskolin and Dibutyryl-cAMP, through the elevation of cAMP levels and subsequent activation of protein kinase A (PKA), can lead to the phosphorylation of CLEC2M. This post-translational modification can activate the protein. Sodium Orthovanadate, by inhibiting protein tyrosine phosphatases, prevents the dephosphorylation of proteins such as CLEC2M, thus maintaining its activated state. Lastly, Brefeldin A disrupts the Golgi apparatus, potentially altering glycosylation patterns and exposing CLEC2M to a broader range of activating glycoprotein ligands.
SEE ALSO...
| Product Name | CAS # | Catalog # | QUANTITY | Price | Citations | RATING |
|---|---|---|---|---|---|---|
A23187 | 52665-69-7 | sc-3591 sc-3591B sc-3591A sc-3591C | 1 mg 5 mg 10 mg 25 mg | $55.00 $131.00 $203.00 $317.00 | 23 | |
Calcium Ionophore A23187 increases intracellular calcium levels, which can activate calcium-dependent signaling pathways. Because C-type lectin domain family 2, member m (CLEC2M) is a calcium-dependent lectin, enhanced calcium levels can directly lead to the activation of CLEC2M by promoting its binding to glycoprotein ligands. | ||||||
Zinc | 7440-66-6 | sc-213177 | 100 g | $48.00 | ||
Zinc Chloride provides zinc ions, which are crucial for the structural integrity of many lectins. Zinc ions can directly activate CLEC2M by maintaining the protein's conformation in a state that is capable of binding to its ligands, which is essential for its function. | ||||||
Manganese(II) chloride beads | 7773-01-5 | sc-252989 sc-252989A | 100 g 500 g | $19.00 $31.00 | ||
Manganese(II) Chloride supplies manganese ions that can activate kinases involved in the phosphorylation of proteins. As phosphorylation can regulate the activity of lectins, this may lead to the activation of CLEC2M through post-translational modifications that enhance its ligand-binding activity. | ||||||
Cobalt(II) chloride | 7646-79-9 | sc-252623 sc-252623A | 5 g 100 g | $64.00 $176.00 | 7 | |
Cobalt(II) Chloride provides cobalt ions that can stabilize the structure of lectins. Cobalt ions can activate CLEC2M by supporting the protein's conformation for effective ligand interaction which is central to its activation and function. | ||||||
Thapsigargin | 67526-95-8 | sc-24017 sc-24017A | 1 mg 5 mg | $136.00 $446.00 | 114 | |
Thapsigargin is a potent inhibitor of the endoplasmic reticulum Ca2+ ATPase, leading to an increase in cytosolic calcium levels. Elevated calcium can activate CLEC2M by promoting its calcium-dependent binding to specific carbohydrate structures. | ||||||
PMA | 16561-29-8 | sc-3576 sc-3576A sc-3576B sc-3576C sc-3576D | 1 mg 5 mg 10 mg 25 mg 100 mg | $41.00 $132.00 $214.00 $500.00 $948.00 | 119 | |
PMA activates protein kinase C (PKC), which can phosphorylate various proteins including lectins. PKC-mediated phosphorylation can lead to the activation of CLEC2M by inducing conformational changes that enhance its ligand-binding capability. | ||||||
Ionomycin | 56092-82-1 | sc-3592 sc-3592A | 1 mg 5 mg | $78.00 $270.00 | 80 | |
Ionomycin is a calcium ionophore that raises intracellular calcium levels, similar to A23187. Increased intracellular calcium can activate CLEC2M by promoting its binding to ligands, which is a calcium-dependent process. | ||||||
Dibutyryl-cAMP | 16980-89-5 | sc-201567 sc-201567A sc-201567B sc-201567C | 20 mg 100 mg 500 mg 10 g | $47.00 $136.00 $492.00 $4552.00 | 74 | |
Dibutyryl-cAMP is a cell-permeable cAMP analog that activates PKA. Activated PKA can phosphorylate CLEC2M, potentially leading to its activation by enhancing its ability to bind to ligands. | ||||||
Sodium Orthovanadate | 13721-39-6 | sc-3540 sc-3540B sc-3540A | 5 g 10 g 50 g | $49.00 $57.00 $187.00 | 142 | |
Sodium Orthovanadate is an inhibitor of protein tyrosine phosphatases. By preventing dephosphorylation, this chemical can lead to the sustained phosphorylation and consequent activation of CLEC2M, assuming CLEC2M activity is regulated by phosphorylation. | ||||||
Brefeldin A | 20350-15-6 | sc-200861C sc-200861 sc-200861A sc-200861B | 1 mg 5 mg 25 mg 100 mg | $31.00 $53.00 $124.00 $374.00 | 25 | |
Brefeldin A disrupts Golgi apparatus function, which can lead to altered glycosylation patterns on proteins. As glycosylation is important for lectin function, this disruption may activate CLEC2M by exposing it to a wider range of glycoprotein ligands or altering its glycosylation state, thus potentially enhancing its activity. | ||||||