WASP Antibody (B-12): sc-398477

WASP Antibody (B-12) is a mouse monoclonal IgG1 antibody that detects WASP in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). WASP, or Wiskott-Aldrich syndrome protein, plays a crucial role in the regulation of the actin cytoskeleton, which is essential for various cellular processes, including cell shape, motility, and division. The proper functioning of WASP is particularly important in immune cells, where WASP facilitates the formation of actin-rich structures necessary for effective immune responses. Mutations in the WASP gene lead to the Wiskott-Aldrich syndrome, characterized by immunodeficiency and increased susceptibility to infections, highlighting WASP′s vital role in maintaining immune system integrity. WASP (B-12) antibody contains a unique structure that includes a Cdc42/Rac interacting domain, which is critical for WASP′s function as a downstream effector of Cdc42, a small GTPase involved in cytoskeletal dynamics. By utilizing anti-WASP antibody (B-12), researchers can gain insights into WASP′s involvement in cytoskeletal organization and implications in various diseases, making WASP (B-12) antibody an invaluable tool for studying the underlying mechanisms of Wiskott-Aldrich syndrome and related disorders.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

WASP Antibody (B-12) References:

1. Neural Wiskott-Aldrich syndrome protein (N-WASP) is the specific ligand for Shigella VirG among the WASP family and determines the host cell type allowing actin-based spreading.  |  Suzuki, T., et al. 2002. Cell Microbiol. 4: 223-33. PMID: 11952639
2. Regulation of actin cytoskeleton by mDab1 through N-WASP and ubiquitination of mDab1.  |  Suetsugu, S., et al. 2004. Biochem J. 384: 1-8. PMID: 15361067
3. Association of Cdc42/N-WASP/Arp2/3 signaling pathway with Golgi membranes.  |  Matas, OB., et al. 2004. Traffic. 5: 838-46. PMID: 15479449
4. The rate of N-WASP exchange limits the extent of ARP2/3-complex-dependent actin-based motility.  |  Weisswange, I., et al. 2009. Nature. 458: 87-91. PMID: 19262673
5. Dynamic interaction of amphiphysin with N-WASP regulates actin assembly.  |  Yamada, H., et al. 2009. J Biol Chem. 284: 34244-56. PMID: 19759398
6. The non-redundant role of N-WASP in podosome-mediated matrix degradation in macrophages.  |  Nusblat, LM., et al. 2011. Eur J Cell Biol. 90: 205-12. PMID: 20732727
7. WIP provides an essential link between Nck and N-WASP during Arp2/3-dependent actin polymerization.  |  Donnelly, SK., et al. 2013. Curr Biol. 23: 999-1006. PMID: 23707428
8. N-WASP is required for B-cell-mediated autoimmunity in Wiskott-Aldrich syndrome.  |  Volpi, S., et al. 2016. Blood. 127: 216-20. PMID: 26468226
9. VCA nanobodies target N-WASp to reduce invadopodium formation and functioning.  |  Hebbrecht, T., et al. 2017. PLoS One. 12: e0185076. PMID: 28938008
10. Rac1/WAVE2 and Cdc42/N-WASP Participation in Actin-Dependent Host Cell Invasion by Extracellular Amastigotes of Trypanosoma cruzi.  |  Bonfim-Melo, A., et al. 2018. Front Microbiol. 9: 360. PMID: 29541069

The preferred WASP antibody is WASP Antibody (B-9): sc-13139.