VILIP-1 CRISPR/Cas9 KO Plasmid (h): sc-401100
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- VILIP-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the VILIP-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: VILIP-1 Antibody (2F1-E3): sc-293209
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
VILIP-1 CRISPR/Cas9 KO Plasmid (h) | sc-401100 | 20 µg | $397.00 |
Overview
VSNL1 encodes visinin-like protein 1 (VILIP-1), a neuronal calcium sensor of the EF-hand family that undergoes Ca2+-dependent conformational changes and myristoyl switch-mediated membrane association. VILIP-1 couples intracellular calcium transients to regulation of signal transduction, influencing cAMP/cGMP-linked pathways, synaptic plasticity, neurite outgrowth, and activity-dependent gene expression. In human tissues it is enriched in the nervous system and is commonly used as a marker of neuronal state and calcium signaling dynamics. Altered VSNL1/VILIP-1 expression and calcium homeostasis have been associated with neurodegenerative and neuropsychiatric disease biology, supporting mechanistic studies of neuronal vulnerability and stress responses.
VILIP-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the VSNL1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the VSNL1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the VSNL1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish VILIP-1 protein expression.
This CRISPR knockout system enables efficient generation of VSNL1-deficient cell models for investigation of VILIP-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting VSNL1 exon(s) critical for VILIP-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple VSNL1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by VILIP-1 CRISPR/Cas9 KO Plasmid (h) and VILIP-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the VSNL1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by VILIP-1 HDR Plasmid (h) and VILIP-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by VSNL1 homology arms to support homology-directed repair at defined VSNL1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.