TET2 Double Nickase Plasmid (h): sc-400545-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- TET2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- TET2 Double Nickase Plasmid (h) and TET2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting TET2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: TET2 Antibody (C-7): sc-398535
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
TET2 Double Nickase Plasmid (h) | sc-400545-NIC | 20 µg | $410.00 | |||
TET2 Double Nickase Plasmid (h2) | sc-400545-NIC-2 | 20 µg | $410.00 |
TET2 encodes a Fe(II)/2-oxoglutarate–dependent dioxygenase that catalyzes oxidation of 5-methylcytosine to 5-hydroxymethylcytosine and further oxidized derivatives, promoting active and passive DNA demethylation. Through epigenetic regulation of enhancers and gene bodies, TET2 helps coordinate hematopoietic differentiation, immune cell fate decisions, and transcriptional programs linked to chromatin accessibility. TET2 activity intersects with DNA repair and replication-associated demethylation processes and is modulated by metabolic cues that influence dioxygenase cofactor availability. Disruption of TET2-mediated methylation dynamics is frequently associated with altered myeloid and lymphoid lineage homeostasis and is widely studied in the context of clonal hematopoiesis and hematologic malignancy mechanisms.
TET2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the TET2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within TET2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt TET2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of TET2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.