p53 Antibody (pAb 122): sc-56182

p53 Antibody (pAb 122) is an IgG2b kappa light chain mouse monoclonal antibody that specifically binds to the human p53 protein at the epitope encompassing amino acids 370-378, which is part of a conserved, denaturation-resistant determinant within the tetramerization domain of p53. This specificity allows the anti-p53 antibody (pAb 122) to detect both wild-type and mutant forms of p53 from human, mouse, and rat origins using western blotting, immunoprecipitation, immunofluorescence, and flow cytometry. The p53 monoclonal antibody (pAb 122) is available in non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing flexibility for diverse experimental applications. The p53 protein is a pivotal tumor suppressor protein containing DNA-binding, oligomerization, and transcription activation domains, functioning to upregulate growth arrest and apoptosis-related genes in response to cellular stress signals. p53 influences programmed cell death, cell differentiation, and cell cycle control mechanisms. While predominantly localized in the nucleus, p53 can be transported to the cytoplasm by its negative regulator MDM2, an E3 ubiquitin ligase that targets p53 for proteasomal degradation through polyubiquitination. The activity of p53 is finely regulated through post-translational modifications such as phosphorylation and acetylation, which modulate its DNA-binding affinity and transcriptional activity. Mutations in the DNA-binding domain (amino acids 110-286) often impair p53′s ability to bind DNA and are associated with numerous human cancers. The ability of p53 monoclonal mouse antibody (pAb 122) to recognize a denaturation-resistant epitope makes it an invaluable tool for detecting p53 under various experimental conditions, including those involving denatured proteins.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

p53 Antibody (pAb 122) References:

1. Functions of the MDM2 oncoprotein.  |  Freedman, DA., et al. 1999. Cell Mol Life Sci. 55: 96-107. PMID: 10065155
2. Structural biology of the tumor suppressor p53 and cancer-associated mutants.  |  Joerger, AC. and Fersht, AR. 2007. Adv Cancer Res. 97: 1-23. PMID: 17419939
3. A DNA binding domain is contained in the C-terminus of wild type p53 protein.  |  Foord, OS., et al. 1991. Nucleic Acids Res. 19: 5191-8. PMID: 1923804
4. Mutant p53 protein, master regulator of human malignancies: a report on the Fifth Mutant p53 Workshop.  |  Blandino, G., et al. 2012. Cell Death Differ. 19: 180-3. PMID: 22095277
5. Cancer therapeutic approach based on conformational stabilization of mutant p53 protein by small peptides.  |  Tal, P., et al. 2016. Oncotarget. 7: 11817-37. PMID: 26943582
6. Disposable Amperometric Immunosensor for the Determination of Human P53 Protein in Cell Lysates Using Magnetic Micro-Carriers.  |  Pedrero, M., et al. 2016. Biosensors (Basel). 6: PMID: 27879639
7. Increase in p53 protein half-life in mouse keratinocytes following UV-B irradiation.  |  Liu, M., et al. 1994. Carcinogenesis. 15: 1089-92. PMID: 8020138
8. Protein synthesis-dependent cytoplasmic translocation of p53 protein after serum stimulation of growth-arrested MCF-7 cells.  |  Takahashi, K., et al. 1993. Mol Carcinog. 8: 58-66. PMID: 8352892
9. Human p53 binds DNA as a protein homodimer but monomeric variants retain full transcription transactivation activity.  |  Tarunina, M. and Jenkins, JR. 1993. Oncogene. 8: 3165-73. PMID: 8414520
10. The MDM2 oncoprotein is overexpressed in rhabdomyosarcoma cell lines and stabilizes wild-type p53 protein.  |  Keleti, J., et al. 1996. Am J Pathol. 149: 143-51. PMID: 8686737

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p53 Antibody (pAb 122) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-p53 Antibody (pAb 122) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p53 Antibody (pAb 122) Specifications

Alternate Product Name: TP53

Product Clone Name: pAb 122

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids: 370-378

Epitope Target: p53

Epitope Species: mouse

Product Reactivity/Detection: a conserved, denaturation-resistant determinant of the p53 protein

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for p53 Antibody (pAb 122): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p53 antibody is p53 Antibody (DO-1): sc-126.