Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
p22-phox CRISPR/Cas9 KO Plasmid (h): sc-400325
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- p22-phox CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the p22-phox genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: p22-phox Antibody (E-11): sc-271968
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p22-phox CRISPR/Cas9 KO Plasmid (h) | sc-400325 | 20 µg | $397.00 |
Overview
CYBA encodes p22-phox, an essential membrane subunit of the phagocyte NADPH oxidase (NOX2) complex that stabilizes gp91-phox/NOX2 and supports assembly of the active oxidase at phagosomal and plasma membranes. By enabling electron transfer to molecular oxygen, p22-phox is required for regulated reactive oxygen species (ROS) production that contributes to antimicrobial defense and redox-dependent signaling in innate immune cells. CYBA-dependent ROS influences pathways linked to phagocytosis, inflammatory mediator production, and oxidative burst dynamics. Genetic disruption or altered expression of CYBA is associated with impaired NOX activity and is studied in the context of chronic granulomatous disease–like phenotypes, inflammatory dysregulation, and oxidative stress–related cellular responses.
p22-phox CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CYBA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CYBA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CYBA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish p22-phox protein expression.
This CRISPR knockout system enables efficient generation of CYBA-deficient cell models for investigation of p22-phox signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CYBA exon(s) critical for p22-phox function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CYBA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by p22-phox CRISPR/Cas9 KO Plasmid (h) and p22-phox CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CYBA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by p22-phox HDR Plasmid (h) and p22-phox HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CYBA homology arms to support homology-directed repair at defined CYBA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.