Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histone Deacetylase 5 (HDAC5) Double Nickase Plasmid (h): sc-400659-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone Deacetylase 5 (HDAC5) Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Histone Deacetylase 5 (HDAC5) Double Nickase Plasmid (h) and Histone Deacetylase 5 (HDAC5) Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HDAC5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone Deacetylase 5 (HDAC5) Antibody (C-11): sc-133225
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone Deacetylase 5 (HDAC5) Double Nickase Plasmid (h) | sc-400659-NIC | 20 µg | $410.00 | |||
Histone Deacetylase 5 (HDAC5) Double Nickase Plasmid (h2) | sc-400659-NIC-2 | 20 µg | $410.00 |
HDAC5 encodes histone deacetylase 5, a class IIa HDAC that modulates chromatin accessibility and transcriptional programs by partnering with corepressor complexes and deacetylating histone and non-histone substrates. Its activity is dynamically regulated by phosphorylation-dependent nucleo-cytoplasmic shuttling, linking calcium and kinase signaling to gene expression control. HDAC5 is a key node in pathways governing muscle differentiation, neuronal plasticity, and stress-responsive transcription, including MEF2-dependent networks. Dysregulated HDAC5 function and localization have been implicated in aberrant epigenetic states associated with cancer biology, cardiac remodeling, and neuropsychiatric phenotypes, supporting its use in mechanistic studies of chromatin regulation.
Histone Deacetylase 5 (HDAC5) Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HDAC5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HDAC5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HDAC5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HDAC5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.