GDPD3 CRISPR/Cas9 KO Plasmid (h): sc-405288
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GDPD3 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GDPD3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GDPD3 CRISPR/Cas9 KO Plasmid (h) | sc-405288 | 20 µg | $397.00 |
Overview
GDPD3 (glycerophosphodiester phosphodiesterase domain containing 3) is a human glycerophosphodiesterase implicated in phospholipid catabolism and glycerophospholipid remodeling, processes that shape membrane composition and lipid-derived signaling. By hydrolyzing glycerophosphodiesters, GDPD3 can influence cellular pools of metabolites that support membrane trafficking, organelle homeostasis, and broader lipid metabolic networks. Altered regulation of glycerophospholipid metabolism is frequently connected to changes in proliferation, stress adaptation, and immune-related signaling, making GDPD3 a relevant node for mechanistic studies in metabolic reprogramming. Expression and activity changes in lipid enzymes, including GDPD3, have been reported across diverse disease-relevant contexts, supporting investigation of its contribution to dysregulated lipid homeostasis.
GDPD3 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GDPD3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GDPD3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GDPD3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GDPD3 protein expression.
This CRISPR knockout system enables efficient generation of GDPD3-deficient cell models for investigation of GDPD3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GDPD3 exon(s) critical for GDPD3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GDPD3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GDPD3 CRISPR/Cas9 KO Plasmid (h) and GDPD3 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GDPD3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GDPD3 HDR Plasmid (h) and GDPD3 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GDPD3 homology arms to support homology-directed repair at defined GDPD3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.