ELOVL5 Double Nickase Plasmid (h): sc-417429-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ELOVL5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- ELOVL5 Double Nickase Plasmid (h) and ELOVL5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ELOVL5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ELOVL5 Antibody (B-3): sc-374138
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ELOVL5 Double Nickase Plasmid (h) | sc-417429-NIC | 20 µg | $410.00 | |||
ELOVL5 Double Nickase Plasmid (h2) | sc-417429-NIC-2 | 20 µg | $410.00 |
ELOVL5 encodes an elongase that catalyzes the extension of long-chain polyunsaturated fatty acids to very-long-chain fatty acids, supporting synthesis of membrane lipids and lipid-derived signaling mediators. It functions in endoplasmic reticulum lipid metabolism and intersects with pathways regulating phospholipid remodeling, eicosanoid and docosanoid precursor availability, and cellular stress responses linked to membrane composition. Altered ELOVL5 activity has been associated with dysregulated lipid homeostasis in metabolic and inflammatory contexts and has been implicated in neurodegenerative phenotypes where fatty acid composition influences neuronal function. As a result, ELOVL5 is widely studied in systems biology of lipid metabolism, membrane dynamics, and lipidomics-based mechanism research.
ELOVL5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ELOVL5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ELOVL5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ELOVL5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ELOVL5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.