Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CGGBP1 CRISPR/Cas9 KO Plasmid (h): sc-407049
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CGGBP1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CGGBP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CGGBP1 Antibody (G-8): sc-376482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CGGBP1 CRISPR/Cas9 KO Plasmid (h) | sc-407049 | 20 µg | $397.00 |
Overview
CGGBP1 (CGG triplet repeat-binding protein 1) is a nuclear DNA-binding factor implicated in regulation of transcription and maintenance of genome stability through interactions with repeat-rich genomic regions. It has been linked to control of cell cycle progression, chromatin-associated processes, and cellular responses to replication stress, supporting orderly DNA replication and mitotic fidelity. CGGBP1 activity is frequently studied in the context of epigenetic regulation and DNA damage signaling pathways that shape cell proliferation and stress adaptation. Altered CGGBP1 expression or function has been associated with dysregulated growth phenotypes and features relevant to cancer biology, including aberrant transcriptional programs and genomic instability.
CGGBP1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CGGBP1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CGGBP1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CGGBP1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CGGBP1 protein expression.
This CRISPR knockout system enables efficient generation of CGGBP1-deficient cell models for investigation of CGGBP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CGGBP1 exon(s) critical for CGGBP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CGGBP1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CGGBP1 CRISPR/Cas9 KO Plasmid (h) and CGGBP1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CGGBP1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CGGBP1 HDR Plasmid (h) and CGGBP1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CGGBP1 homology arms to support homology-directed repair at defined CGGBP1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.