cadherin-8 Double Nickase Plasmid (h): sc-401576-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cadherin-8 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- cadherin-8 Double Nickase Plasmid (h) and cadherin-8 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDH8. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cadherin-8 Double Nickase Plasmid (h) | sc-401576-NIC | 20 µg | $410.00 | |||
cadherin-8 Double Nickase Plasmid (h2) | sc-401576-NIC-2 | 20 µg | $410.00 |
CDH8 encodes cadherin-8, a calcium-dependent cell–cell adhesion molecule of the classical cadherin family that supports selective neuronal recognition and stable intercellular junctions. Through homophilic binding and coupling to catenins, cadherin-8 contributes to adherens junction organization, actin cytoskeleton remodeling, and contact-dependent signaling that influences neurite outgrowth and synaptic connectivity. CDH8 activity intersects with pathways governing tissue morphogenesis and neural circuit assembly, where precise adhesion dynamics are required for normal development. Altered CDH8 expression or variation has been associated with neurodevelopmental phenotypes, making it a useful target for studying mechanisms linking adhesion to neural network formation and related disease biology.
cadherin-8 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDH8 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDH8. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDH8 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDH8-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.