Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Adenosine A1-R Double Nickase Plasmid (h): sc-401241-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Adenosine A1-R Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Adenosine A1-R Double Nickase Plasmid (h) and Adenosine A1-R Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting ADORA1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Adenosine A1-R Antibody (D-5): sc-514337
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Adenosine A1-R Double Nickase Plasmid (h) | sc-401241-NIC | 20 µg | $410.00 | |||
Adenosine A1-R Double Nickase Plasmid (h2) | sc-401241-NIC-2 | 20 µg | $410.00 |
ADORA1 encodes the human adenosine A1 receptor (Adenosine A1-R), a Gi/o-coupled GPCR that senses extracellular adenosine to modulate intracellular cAMP, ion channel activity, and MAPK signaling. A1-R engagement typically dampens neurotransmitter release and influences cellular excitability, metabolic homeostasis, and responses to hypoxia and inflammation through purinergic signaling networks. ADORA1 signaling intersects with adenylyl cyclase/PKA pathways and can shape downstream transcriptional programs via ERK and related kinase cascades. Dysregulated adenosine–A1-R tone has been implicated in diverse disease-relevant processes including neurological excitability, ischemia-related stress responses, and inflammatory regulation, supporting mechanistic studies in neurobiology and immunometabolism.
Adenosine A1-R Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the ADORA1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within ADORA1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt ADORA1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of ADORA1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.