Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Wnt-8a Antibody (14-3) is a high quality monoclonal Wnt-8a antibody (also designated WNT8A antibody, or WNT8D antibody) suitable for the detection of the Wnt-8a protein of mouse origin. Wnt-8a Antibody (14-3) is available as the non-conjugated anti-Wnt-8a antibody. The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene "wingless" and to its vertebrate ortholog, Int-1, a mouse protooncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins, which have features typical of secreted growth factors. Wnt-8b was named on the basis of the very high sequence similarity (approximately 90% identity) of the inferred protein to those encoded by the Xenopus and zebrafish Wnt-8b genes. The human and mouse ex-pression patterns appear to be identical and are restricted to the developing brain, with the great majority of expression being found in the developing forebrain. Expression in the developing hippocampus may suggest a role for Wnt-8b in patterning of this region. The gene which encodes Wnt-8b maps to human chromosome 10q24.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
How much sample do you recommend loading on the gel for Western blot detection using Wnt-8a (14-3): sc-80459?
Preguntado por: Randy McDonald
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference.
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