USP21 Double Nickase Plasmid (h): sc-404690-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- USP21 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- USP21 Double Nickase Plasmid (h) and USP21 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting USP21. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: USP21 Antibody (B-9): sc-515911
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
USP21 Double Nickase Plasmid (h) | sc-404690-NIC | 20 µg | $410.00 | |||
USP21 Double Nickase Plasmid (h2) | sc-404690-NIC-2 | 20 µg | $410.00 |
Human USP21 encodes a deubiquitinating enzyme in the ubiquitin-specific protease family that removes ubiquitin chains from protein substrates to modulate their stability, localization, and signaling output. USP21 activity interfaces with ubiquitin-dependent control of innate immune signaling, transcriptional regulation, and proteostasis, influencing pathway amplitude and duration through reversible deubiquitination. By shaping ubiquitin dynamics, USP21 can affect cell-state decisions such as inflammatory responses and stress adaptation, processes frequently altered in cancer and immune-associated pathologies. Dysregulated USP21 expression or activity has been linked to aberrant signaling and chromatin-associated regulation, making it a relevant target for mechanistic studies of ubiquitin pathways.
USP21 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the USP21 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within USP21. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt USP21 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of USP21-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.