Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Tenascin-R CRISPR/Cas9 KO Plasmid (m): sc-423465
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Tenascin-R CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Tenascin-R genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Tenascin-R Antibody (A-2): sc-376341
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Tenascin-R CRISPR/Cas9 KO Plasmid (m) | sc-423465 | 20 µg | $397.00 |
Overview
Tnr encodes Tenascin-R, an extracellular matrix glycoprotein enriched in the central nervous system that modulates cell–cell and cell–matrix interactions during neural development and circuit maturation. Tenascin-R contributes to neurite outgrowth, axon guidance, synaptic stabilization, and myelination, in part through interactions with adhesion molecules and proteoglycans that shape the perineuronal net. By influencing extracellular signaling and structural plasticity, it impacts processes such as neuronal connectivity and activity-dependent remodeling. Altered Tenascin-R function or expression has been associated with neurodevelopmental and neuropsychiatric phenotypes, making it relevant for studying mechanisms underlying synaptic organization and network excitability in mouse models.
Tenascin-R CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Tnr gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Tnr together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Tnr open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Tenascin-R protein expression.
This CRISPR knockout system enables efficient generation of Tnr-deficient cell models for investigation of Tenascin-R signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Tnr exon(s) critical for Tenascin-R function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Tnr genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Tenascin-R CRISPR/Cas9 KO Plasmid (m) and Tenascin-R CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Tnr locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Tenascin-R HDR Plasmid (m) and Tenascin-R HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Tnr homology arms to support homology-directed repair at defined Tnr target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.