SP-lyase Double Nickase Plasmid (h): sc-402278-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- SP-lyase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- SP-lyase Double Nickase Plasmid (h) and SP-lyase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting SGPL1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
SP-lyase Double Nickase Plasmid (h) | sc-402278-NIC | 20 µg | $410.00 | |||
SP-lyase Double Nickase Plasmid (h2) | sc-402278-NIC-2 | 20 µg | $410.00 |
SGPL1 encodes human sphingosine-1-phosphate lyase (SP-lyase), an endoplasmic reticulum–associated enzyme that catalyzes the irreversible cleavage of sphingosine-1-phosphate (S1P) to hexadecenal and phosphoethanolamine. By terminating S1P signaling and linking sphingolipid catabolism to phospholipid biosynthesis, SP-lyase regulates lipid homeostasis, membrane composition, and bioactive lipid gradients that influence cell migration, survival, and inflammatory responses. SGPL1 activity intersects with sphingolipid metabolism, ceramide/S1P rheostat dynamics, and downstream GPCR-mediated signaling pathways that shape cellular stress responses and barrier function. Genetic perturbation of SGPL1 is associated with multisystem disorders featuring disrupted sphingolipid profiles, supporting its relevance for mechanistic studies of lipid-driven phenotypes.
SP-lyase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the SGPL1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within SGPL1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt SGPL1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of SGPL1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.