SP-B Antibody (3F340): sc-73048

SP-B Antibody (3F340) is a mouse monoclonal IgG2a antibody that detects pulmonary surfactant protein B (SP-B) in mouse, rat, and human samples through applications such as western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). SP-B is a crucial component of pulmonary surfactant, primarily responsible for reducing surface tension at the air-liquid interface in the alveoli, which is vital for normal respiratory function. SP-B (3F340) monoclonal antibody recognizes a protein that plays a significant role in the absorption of surfactant phospholipids and the stabilization of alveolar structures, thereby preventing alveolar collapse during exhalation. SP-B is predominantly hydrophobic and interacts with lipids to enhance the spreading of surfactant, ensuring efficient gas exchange in the lungs. SP-B (3F340) antibody targets a protein that facilitates the proper functioning of other surfactant proteins, such as SP-C, and is essential for maintaining the integrity of the alveolar surface. SP-B′s importance extends beyond its structural role, as SP-B also contributes to the innate immune response in the lungs, helping to protect against respiratory infections. With SP-B′s ability to bind to lipids and proteins, SP-B is integral to both respiratory mechanics and host defense, making SP-B a critical target for research in pulmonary health and disease.

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SP-B Antibody (3F340) References:

1. Surfactant protein B deficiency: insights into surfactant function through clinical surfactant protein deficiency.  |  Thompson, MW. 2001. Am J Med Sci. 321: 26-32. PMID: 11202476
2. Quantitation of pulmonary surfactant protein SP-B in the absence or presence of phospholipids by enzyme-linked immunosorbent assay.  |  Oviedo, JM., et al. 2001. Anal Biochem. 293: 78-87. PMID: 11373082
3. Lipid-protein interactions of hydrophobic proteins SP-B and SP-C in lung surfactant assembly and dynamics.  |  Pérez-Gil, J. 2001. Pediatr Pathol Mol Med. 20: 445-69. PMID: 11699574
4. Surfactant protein B deficiency in infants with respiratory failure.  |  Wilder, MA. 2004. J Perinat Neonatal Nurs. 18: 61-7. PMID: 15027668
5. Expression of surfactant protein B is dependent on cell density in H441 lung epithelial cells.  |  Fehrholz, M., et al. 2017. PLoS One. 12: e0184556. PMID: 28910374
6. Disulphide Bridges in Surfactant Protein B Analogues Affect Their Activity in Synthetic Surfactant Preparations.  |  Basabe-Burgos, O., et al. 2019. Neonatology. 115: 134-141. PMID: 30453306
7. Mechanism of Lamellar Body Formation by Lung Surfactant Protein B.  |  Sever, N., et al. 2021. Mol Cell. 81: 49-66.e8. PMID: 33242393
8. Disulfide bonds in the SAPA domain of the pulmonary surfactant protein B precursor.  |  Estrada, P., et al. 2022. J Proteomics. 269: 104722. PMID: 36108905
9. A promoterless AAV6.2FF-based lung gene editing platform for the correction of surfactant protein B deficiency.  |  Thomas, SP., et al. 2023. Mol Ther. 31: 3457-3477. PMID: 37805711
10. Human surfactant protein B: structure, function, regulation, and genetic disease.  |  Whitsett, JA., et al. 1995. Physiol Rev. 75: 749-57. PMID: 7480161

The preferred SP-B antibody is SP-B Antibody (F-2): sc-133143.