PXR Antibody (G-11): sc-48403

PXR Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects PXR protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PXR (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pregnane X Receptor (PXR) plays a crucial role in regulating drug metabolism and transport, acting as a key sensor for xenobiotics and endogenous compounds. Located primarily in the nucleus, PXR functions as a transcription factor that, upon activation by ligands such as steroids and certain pharmaceuticals, regulates the expression of genes involved in the metabolism of drugs and toxins. This regulation is vital for maintaining homeostasis and protecting the body from potentially harmful substances. PXR′s ability to form heterodimers with retinoid X receptor (RXR) allows binding to specific DNA sequences, thereby influencing the transcription of target genes. Notably, PXR exists in two isoforms, PXR.1 and PXR.2, which may have distinct regulatory roles, further emphasizing the complexity of cellular signaling pathways. Understanding PXR mechanisms is essential for developing therapeutic strategies that target drug interactions and enhance the efficacy of pharmacological treatments.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PXR Antibody (G-11) References:

1. Small-molecule modulators of PXR and CAR.  |  Chai, SC., et al. 2016. Biochim Biophys Acta. 1859: 1141-1154. PMID: 26921498
2. The steroid and thyroid hormone receptor superfamily.  |  Evans, RM. 1988. Science. 240: 889-95. PMID: 3283939
3. PXR mediates mifepristone-induced hepatomegaly in mice.  |  Yao, XP., et al. 2022. Acta Pharmacol Sin. 43: 146-156. PMID: 33782543
4. The Nuclear Receptor PXR in Chronic Liver Disease.  |  Sayaf, K., et al. 2021. Cells. 11: PMID: 35011625
5. PXR as the tipping point between innate immune response, microbial infections, and drug metabolism.  |  Bautista-Olivier, CD. and Elizondo, G. 2022. Biochem Pharmacol. 202: 115147. PMID: 35714683
6. ncBAF enhances PXR-mediated transcriptional activation in the human and mouse liver.  |  Kurosawa, K., et al. 2023. Biochem Pharmacol. 215: 115733. PMID: 37543347
7. Chemical manipulation of an activation/inhibition switch in the nuclear receptor PXR.  |  Garcia-Maldonado, E., et al. 2024. Nat Commun. 15: 4054. PMID: 38744881
8. The RXR heterodimers and orphan receptors.  |  Mangelsdorf, DJ. and Evans, RM. 1995. Cell. 83: 841-50. PMID: 8521508
9. Steroid hormone receptors: many actors in search of a plot.  |  Beato, M., et al. 1995. Cell. 83: 851-7. PMID: 8521509
10. Dexamethasone responsiveness of a major glucocorticoid-inducible CYP3A gene is mediated by elements unrelated to a glucocorticoid receptor binding motif.  |  Huss, JM., et al. 1996. Proc Natl Acad Sci U S A. 93: 4666-70. PMID: 8643461
11. A signature motif in transcriptional co-activators mediates binding to nuclear receptors.  |  Heery, DM., et al. 1997. Nature. 387: 733-6. PMID: 9192902
12. An orphan nuclear receptor activated by pregnanes defines a novel steroid signaling pathway.  |  Kliewer, SA., et al. 1998. Cell. 92: 73-82. PMID: 9489701

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PXR Antibody (G-11) has 23 citations in a variety of scientific publications.

Primary antibodies like Anti-PXR Antibody (G-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PXR Antibody (G-11) Specifications

Alternate Product Name: Pregnane X receptor

Product Clone Name: G-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 101-260

Epitope Target: PXR

Epitope Species: human

Product Reactivity/Detection: PXR.1 and PXR.2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 23 citations in various scientific publications

Available Conjugates/Forms for PXR Antibody (G-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred PXR antibody is PXR Antibody (H-11): sc-48340.