Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PI 3-kinase p85β CRISPR/Cas9 KO Plasmid (h): sc-401991
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PI 3-kinase p85β CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PI 3-kinase p85β genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PI 3-kinase p85β Antibody (H-1): sc-515646
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PI 3-kinase p85β CRISPR/Cas9 KO Plasmid (h) | sc-401991 | 20 µg | $397.00 |
Overview
PIK3R2 encodes the p85β regulatory subunit of class IA phosphoinositide 3-kinase, which stabilizes and modulates p110 catalytic subunits and links activated receptor tyrosine kinases and adaptor proteins to PI3K signaling. Through control of PIP3 production, PI 3-kinase p85β governs AKT–mTOR and related pathways that regulate cell growth, metabolism, survival, and cytoskeletal dynamics. Perturbation of PI3K pathway regulation is broadly implicated in oncogenic signaling, insulin/IGF responses, and immune cell function. Because p85β influences both basal and stimulus-dependent PI3K activity, it is frequently studied in contexts of signaling feedback, pathway crosstalk, and network rewiring.
PI 3-kinase p85β CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PIK3R2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PIK3R2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PIK3R2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PI 3-kinase p85β protein expression.
This CRISPR knockout system enables efficient generation of PIK3R2-deficient cell models for investigation of PI 3-kinase p85β signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PIK3R2 exon(s) critical for PI 3-kinase p85β function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PIK3R2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PI 3-kinase p85β CRISPR/Cas9 KO Plasmid (h) and PI 3-kinase p85β CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PIK3R2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PI 3-kinase p85β HDR Plasmid (h) and PI 3-kinase p85β HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PIK3R2 homology arms to support homology-directed repair at defined PIK3R2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.