PAOX Double Nickase Plasmid (h): sc-406710-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PAOX Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- PAOX Double Nickase Plasmid (h) and PAOX Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PAOX. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PAOX Double Nickase Plasmid (h) | sc-406710-NIC | 20 µg | $410.00 | |||
PAOX Double Nickase Plasmid (h2) | sc-406710-NIC-2 | 20 µg | $410.00 |
Human PAOX (polyamine oxidase) is a flavin-dependent enzyme that catalyzes the oxidation of N1-acetylspermine and N1-acetylspermidine, contributing to polyamine back-conversion and cellular polyamine homeostasis. Through production of spermidine/putrescine and reactive byproducts such as hydrogen peroxide, PAOX activity links polyamine metabolism to redox balance and oxidative stress signaling. This pathway interfaces with processes including cell proliferation, differentiation, and stress responses via modulation of intracellular polyamine pools. Dysregulation of polyamine catabolism and associated oxidative stress has been studied in contexts such as cancer biology, neurodegeneration, and inflammatory tissue injury, supporting PAOX as a useful target for mechanistic investigation.
PAOX Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PAOX locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PAOX. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PAOX function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PAOX-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.