Net CRISPR/Cas9 KO Plasmid (m): sc-420157
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Net CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Net genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Net CRISPR/Cas9 KO Plasmid (m) | sc-420157 | 20 µg | $397.00 |
Overview
Elk3 encodes the ETS-family transcription factor Net, a context-dependent regulator that integrates MAPK/ERK signaling with serum response factor (SRF) complexes to modulate immediate-early gene programs. Net binds ETS motifs at promoters and enhancers and can act as a transcriptional repressor or activator depending on phosphorylation status and cofactor availability, shaping cell-state transitions linked to proliferation, migration, and differentiation. In mouse systems, Elk3/Net activity is frequently studied in vascular and stromal biology where ETS–SRF circuitry influences angiogenic gene expression and tissue remodeling. Dysregulation of ETS transcriptional networks is implicated in oncogenic signaling, invasive phenotypes, and aberrant vascular responses, making Elk3 a useful node for mechanistic pathway interrogation.
Net CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Elk3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Elk3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Elk3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Net protein expression.
This CRISPR knockout system enables efficient generation of Elk3-deficient cell models for investigation of Net signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Elk3 exon(s) critical for Net function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Elk3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Net CRISPR/Cas9 KO Plasmid (m) and Net CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Elk3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Net HDR Plasmid (m) and Net HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Elk3 homology arms to support homology-directed repair at defined Elk3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.