NALP6 Double Nickase Plasmid (m): sc-430389-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NALP6 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- NALP6 Double Nickase Plasmid (m) and NALP6 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Nlrp6. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NALP6 Double Nickase Plasmid (m) | sc-430389-NIC | 20 µg | $410.00 | |||
NALP6 Double Nickase Plasmid (m2) | sc-430389-NIC-2 | 20 µg | $410.00 |
Mouse Nlrp6 encodes NALP6, a cytosolic NOD-like receptor that functions as an innate immune sensor and regulator of inflammasome-associated signaling. NALP6 influences caspase-1 activation, IL-1 family cytokine maturation, and downstream inflammatory transcriptional programs, linking microbial and danger sensing to epithelial and myeloid immune responses. In the gastrointestinal tract, NLRP6 has been implicated in maintaining mucosal barrier function and shaping host–microbiome interactions, with relevance to models of colitis and intestinal inflammation. Altered NLRP6 activity has also been studied in the context of infection susceptibility and inflammation-driven tissue homeostasis.
NALP6 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Nlrp6 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Nlrp6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Nlrp6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Nlrp6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.