NALP1A CRISPR/Cas9 KO Plasmid (m): sc-431476
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- NALP1A CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the NALP1A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
NALP1A CRISPR/Cas9 KO Plasmid (m) | sc-431476 | 20 µg | $397.00 |
Overview
Mouse Nlrp1a encodes NALP1A, a cytosolic NOD-like receptor that functions as an inflammasome sensor linking detection of cellular stress to innate immune signaling. Upon activation, NALP1A promotes assembly of inflammasome complexes that drive caspase-1 activation and downstream maturation of pro-inflammatory cytokines such as IL-1β and IL-18, coupling danger sensing to pyroptotic cell death programs. This pathway interfaces with NF-κB priming, mitochondrial stress responses, and broader pattern recognition receptor networks that regulate tissue inflammation. Dysregulated inflammasome signaling is implicated in inflammatory and autoimmune phenotypes and is widely studied in the context of host–pathogen interactions and sterile inflammatory triggers.
NALP1A CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Nlrp1a gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Nlrp1a together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Nlrp1a open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish NALP1A protein expression.
This CRISPR knockout system enables efficient generation of Nlrp1a-deficient cell models for investigation of NALP1A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Nlrp1a exon(s) critical for NALP1A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Nlrp1a genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by NALP1A CRISPR/Cas9 KO Plasmid (m) and NALP1A CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Nlrp1a locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by NALP1A HDR Plasmid (m) and NALP1A HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Nlrp1a homology arms to support homology-directed repair at defined Nlrp1a target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.