musculin CRISPR/Cas9 KO Plasmid (h): sc-403242
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- musculin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the musculin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: musculin Antibody (4D7): sc-293482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
musculin CRISPR/Cas9 KO Plasmid (h) | sc-403242 | 20 µg | $397.00 |
Overview
MSC encodes musculin, a basic helix–loop–helix (bHLH) transcription factor that modulates lineage-specifying gene expression programs by binding E-box motifs and cooperating with other bHLH regulators. Musculin is implicated in controlling cell fate decisions, differentiation timing, and transcriptional repression/activation dynamics in developmental and tissue-specific contexts, with downstream effects on proliferation and phenotypic stability. Altered MSC expression has been reported across multiple disease-relevant settings, including cancer-associated transcriptional reprogramming and changes in differentiation state, making it a useful node for studying gene regulatory networks. Functional interrogation of musculin helps clarify how bHLH-driven circuits interface with pathways governing growth control and cellular identity.
musculin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MSC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MSC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MSC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish musculin protein expression.
This CRISPR knockout system enables efficient generation of MSC-deficient cell models for investigation of musculin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MSC exon(s) critical for musculin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MSC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by musculin CRISPR/Cas9 KO Plasmid (h) and musculin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MSC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by musculin HDR Plasmid (h) and musculin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MSC homology arms to support homology-directed repair at defined MSC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.