MFG-E8 Antibody (2Q435): sc-71574
- MFG-E8 Antibody (2Q435) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- raised against human milk fat globule membrane tissue / cell preparation
- recommended for detection of MFG-E8 of human origin by WB, IP, IF, IHC(P) and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for MFG-E8 Antibody (2Q435) for WB and IHC(P) applications. These reagents are now offered in bundles with MFG-E8 Antibody (2Q435) (see ordering information below).
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MFG-E8 Antibody (2Q435) is a mouse monoclonal IgG1 antibody that detects MFG-E8 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). MFG-E8, also known as Milk fat globule-EGF factor 8 or Lactadherin, is a 387 amino acid peripheral membrane protein that plays a crucial role in cellular interactions and signaling. MFG-E8 is primarily located on the membranes of mammary epithelial cells and other tissues, where MFG-E8 serves as a specific ligand for Integrin β5 and Integrin β3, facilitating important processes such as cell attachment and potentially influencing fertilization and apoptosis. The structural composition of MFG-E8, which includes one EGF-like domain and two F5/8 type C domains, is vital for function, as these domains enable MFG-E8 to interact with various cellular components and pathogens. Notably, MFG-E8 has been shown to bind to rotavirus, inhibiting replication and thereby providing a protective mechanism against viral infections. Furthermore, overexpression of MFG-E8 has been linked to breast cancer, indicating potential involvement in tumorigenesis and making MFG-E8 a significant target for cancer research.
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MFG-E8 Antibody (2Q435) References:
- MFG-E8-dependent clearance of apoptotic cells, and autoimmunity caused by its failure. | Hanayama, R., et al. 2006. Curr Dir Autoimmun. 9: 162-72. PMID: 16394660
- MFG-E8 overexpression is associated with poor prognosis in breast cancer patients. | Yu, L., et al. 2019. Pathol Res Pract. 215: 490-498. PMID: 30612778
- Cerebrospinal fluid MFG-E8 as a promising biomarker of amyotrophic lateral sclerosis. | Yang, B., et al. 2020. Neurol Sci. 41: 2915-2920. PMID: 32338335
- Absence of the MFG-E8 gene prevents hypoxia-induced pulmonary hypertension in mice. | Wang, J., et al. 2021. J Cell Physiol. 236: 587-600. PMID: 32592231
- MFG-E8 attenuates inflammation in subarachnoid hemorrhage by driving microglial M2 polarization. | Gao, YY., et al. 2021. Exp Neurol. 336: 113532. PMID: 33245889
- MFG-E8 Plays an Important Role in Attenuating Cerulein-Induced Acute Pancreatitis in Mice. | Bu, HF., et al. 2021. Cells. 10: PMID: 33806041
- MFG-E8 Maintains Cellular Homeostasis by Suppressing Endoplasmic Reticulum Stress in Pancreatic Exocrine Acinar Cells. | Ren, Y., et al. 2021. Front Cell Dev Biol. 9: 803876. PMID: 35096831
- MFG-E8 promotes tendon-bone healing by regualting macrophage efferocytosis and M2 polarization after anterior cruciate ligament reconstruction. | Geng, R., et al. 2022. J Orthop Translat. 34: 11-21. PMID: 35615640
- MFG-E8 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells through GSK3β/β-catenin signaling pathway. | Bai, J., et al. 2023. FASEB J. 37: e22950. PMID: 37144883
- The m6A reader YTHDF1 attenuates fulminant hepatitis via MFG-E8 translation in an m6A dependent manner. | Ke, MY., et al. 2023. Int J Biol Sci. 19: 3987-4003. PMID: 37564203
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MFG-E8 Antibody (2Q435) | sc-71574 | 200 µg/ml | $322.00 | |||
MFG-E8 Antibody (2Q435): m-IgG Fc BP-HRP Bundle | sc-539377 | 200 µg Ab; 10 µg BP | $361.00 | |||
MFG-E8 Antibody (2Q435): m-IgG1 BP-HRP Bundle | sc-541445 | 200 µg Ab; 20 µg BP | $361.00 |