Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
MCM5 Double Nickase Plasmid (h): sc-401980-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MCM5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- MCM5 Double Nickase Plasmid (h) and MCM5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting MCM5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: MCM5 Antibody (E-10): sc-165994
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MCM5 Double Nickase Plasmid (h) | sc-401980-NIC | 20 µg | $410.00 | |||
MCM5 Double Nickase Plasmid (h2) | sc-401980-NIC-2 | 20 µg | $410.00 |
MCM5 encodes a core subunit of the minichromosome maintenance (MCM2–7) helicase complex that licenses replication origins and drives DNA unwinding during S phase. By coordinating replication initiation, fork progression, and replisome stability, MCM5 contributes to genome integrity and orderly cell-cycle transitions through S-phase and G2/M checkpoints. Dysregulated MCM5 expression or altered replication licensing is associated with replication stress, chromosomal instability, and proliferative phenotypes observed across multiple cancer contexts. As a replication factor, MCM5 is frequently used to interrogate DNA replication timing, checkpoint signaling, and mechanisms that couple replication to DNA damage responses.
MCM5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the MCM5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within MCM5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt MCM5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of MCM5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.