HCN3 Double Nickase Plasmid (h): sc-407361-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- HCN3 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- HCN3 Double Nickase Plasmid (h) and HCN3 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting HCN3. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: HCN3 Antibody (TLL6C5): sc-58621
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
HCN3 Double Nickase Plasmid (h) | sc-407361-NIC | 20 µg | $410.00 | |||
HCN3 Double Nickase Plasmid (h2) | sc-407361-NIC-2 | 20 µg | $410.00 |
HCN3 encodes a hyperpolarization-activated cyclic nucleotide–gated channel that contributes to the Ih current by conducting mixed Na⁺/K⁺ inward current during membrane hyperpolarization. By integrating voltage dependence with modulation by cyclic nucleotides, HCN3 helps regulate resting membrane potential, rhythmic excitability, and responsiveness to neuromodulatory signaling in excitable cell types. The channel participates in electrophysiological processes linked to pacemaker-like activity and neuronal firing patterns and interfaces with cAMP-dependent pathways that tune membrane conductance. Altered HCN channel activity has been associated with dysregulated excitability phenotypes relevant to arrhythmia- and seizure-related research contexts, supporting mechanistic studies of ion channel function in disease-relevant cellular models.
HCN3 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the HCN3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within HCN3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt HCN3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of HCN3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.