Date published: 2025-9-11

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GFP Anticuerpo (1A5): sc-101536

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Fichas Técnicas
  • GFP Anticuerpo 1A5 es un monoclonal de rata IgG1, ver las 29 publicaciones, proporcionado 200 µg/ml
  • criogenia frente al GFP recombinante
  • recomendado para detectar GFP and GFP fusion proteins medainte WB, IP, IF y ELISA
  • Actualmente, aún no hemos completado la identificación de los reactivos de detección secundaria preferidos para GFP Anticuerpo (1A5). Este trabajo está en progreso.

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La proteína verde fluorescente (GFP) fue originalmente identificada como una proteína involucrada en la bioluminiscencia de la medusa Aequorea victoria. El ADNc de GFP produce un producto fluorescente cuando se expresa en células procariotas, sin necesidad de sustratos o cofactores exógenos, lo que hace que GFP sea una herramienta útil para monitorear la expresión génica y la localización de proteínas in vivo. Se han desarrollado varios mutantes de GFP, incluido EGFP, que fluoresce con mayor intensidad que el GFP de tipo salvaje. Su máximo de excitación desplazado es más favorable para la citometría de flujo y la microscopía de fluorescencia, así como para aplicaciones de doble etiquetado. GFP se utiliza ampliamente en vectores de expresión como una etiqueta de fusión de proteínas, lo que permite la expresión y el monitoreo de proteínas heterólogas fusionadas a GFP.

Para uso exclusivo en Investigación No está diseñado para para Diagnóstico ó Terapia.

Información sobre pedidos

Nombre del productoNúmero de catálogoUNIDADPrecioCANTIDADFavoritos

GFP Anticuerpo (1A5)

sc-101536
200 µg/ml
$316.00

Does this antibody(GFP Antibody (1A5)) works for venus? If not, do you have any idea which GFP antibody here can recognize Venus?

Preguntado por: Liu M
Thank you for your question. Please contact our Technical Service Department with the specific protein sequence for Venus, and we can perform sequence analysis of our GFP antibody antigens to see if any of them may also detect this protein. We do not have any data or citations for this GFP antibody specifically mentioning reactivity with Venus.
Respondida por: Technical Support
Fecha de publicación: 2020-03-03

Could you tell me which regions or amino acid of the GFP protein is targeted by this antibody?

Preguntado por: jfoul78
Thank you for your question. GFP Antibody (1A5) is a rat monoclonal raised against recombinant GFP. Unfortunately, the exact sequence used as an immunogen is not known, nor is the epitope recognized by this specific clone. We do offer a broad variety of GFP antibodies where this information is available. For example, our GFP Antibody (C-2): sc-390394 is specific for an epitope mapping between amino acids 167-182 within an internal region of GFP of Aequorea victoria origin. Please contact our Technical Service Department for help in choosing the best antibody for your research needs. Please call us toll-free at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat.
Respondida por: Technical Support
Fecha de publicación: 2017-02-03

I would like to use GFP Antibody (1A5): sc-101536 for double staining with another mouse monoclonal primary antibody. Is this possible?

Preguntado por: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-101536 is not available directly conjugated, but we have an alternative GFP antibody (sc-9996) that is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
Respondida por: Technical Support 7
Fecha de publicación: 2017-01-24

I am looking for a GFP antibody that was raised against the amino acid sequence 215-230 (RDHMVL LEFVTAAGIT). Do you have any antibody like this? Thanks!

Preguntado por: Anette
Thank you for your question. Most of our GFP antibodies were raised against full length recombinant GFP, which includes this portion of the sequence that you have specified. However, the exact epitope recognized by each monoclonal antibody has not been determined. If you would be interested in testing a complimentary sample size of our mouse monoclonal antibody, GFP (B-2): sc-9996, to see if it would work for your purposes, please contact our Technical Service Department. Please call us directly at (800) 457-3801 option 2, email scbt@scbt.com, or use our live chat.
Respondida por: Technical Support
Fecha de publicación: 2017-01-19
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  • QUESTIONS, PRODUCT
Rated 1 de 5 por de Did not work in texted tissue samplesI got a free sample and tested it according to th eworking lab protocol.There was no signal in the blot.
Fecha de publicación: 2018-04-09
Rated 5 de 5 por de Positive WB and IFAntibody is positive for both westren blot and IF staining
Fecha de publicación: 2017-02-05
Rated 5 de 5 por de Reliable antibodyGood choice if you're looking for a reliable GFP antibody to use in WB and IF.
Fecha de publicación: 2017-01-11
Rated 5 de 5 por de Strong bands at 1:1000 dilutionStrong bands at 1:1000 dilution.
Fecha de publicación: 2015-09-10
Rated 5 de 5 por de Worked well in WBWorked well in WB, no complaints.
Fecha de publicación: 2015-05-27
Rated 5 de 5 por de Good antibody for western blot analysis ofGood antibody for western blot analysis of GFP expression in 293T cell lysate. -SCBT QC
Fecha de publicación: 2015-04-07
Rated 5 de 5 por de Work well for IF staining of COS cellsWork well for IF staining of COS cells. -SCBT QC
Fecha de publicación: 2015-02-01
Rated 5 de 5 por de Generated publishable data on Hela cellsGenerated publishable data on Hela cells with this antibody. -SCBT Publication Review
Fecha de publicación: 2013-11-23
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GFP Anticuerpo (1A5) clasificado 4.5 de 5 por 8.
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