GCDH CRISPR/Cas9 KO Plasmid (h): sc-405887
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- GCDH CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the GCDH genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
GCDH CRISPR/Cas9 KO Plasmid (h) | sc-405887 | 20 µg | $397.00 |
Overview
Human GCDH encodes glutaryl‑CoA dehydrogenase, a mitochondrial flavoprotein that catalyzes the oxidative decarboxylation of glutaryl‑CoA to crotonyl‑CoA during lysine, hydroxylysine, and tryptophan catabolism. This reaction links amino acid degradation to mitochondrial energy metabolism and redox homeostasis through FAD-dependent electron transfer. GCDH activity influences acyl‑CoA and organic acid balance, shaping mitochondrial stress responses and metabolic remodeling under nutrient flux. Loss-of-function variants are associated with glutaric acidemia type I, and GCDH is frequently studied in the context of neurometabolic vulnerability, mitochondrial dysfunction, and organic acid accumulation.
GCDH CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the GCDH gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the GCDH together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the GCDH open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish GCDH protein expression.
This CRISPR knockout system enables efficient generation of GCDH-deficient cell models for investigation of GCDH signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting GCDH exon(s) critical for GCDH function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple GCDH genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by GCDH CRISPR/Cas9 KO Plasmid (h) and GCDH CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the GCDH locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by GCDH HDR Plasmid (h) and GCDH HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by GCDH homology arms to support homology-directed repair at defined GCDH target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.