G α i1 para análisis funcionales de las repuestas celulares a Heterotrimeric G Protein
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Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter), whereas the effectors (i.e. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein a, b and g subunits are encoded by at least sixteen, four and seven different genes, respectively. The a subunits bind to and hydrolyze GTP. G protein complexes expressed in different tissues contain distinct a, b and g subunits. Preferential associations between members of subunit families increase G protein functional diversity. Most interest in G proteins has been focused on their α subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Gα subunits have been identified; these include Gs, Gi, Gq and Ga 12/13. The Gi class comprises all the known a subunits that are susceptible to pertussis toxin modifications, including Gα i-1, Gα i-2, Gα i-3, Gα o, Gα t1, Gα t2, Gα z and Gα gust. Of these, the three Ga i subtypes function to open atrial potassium channels.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What is the recommended fixation method for immunofluorescence staining with Gα i-1 (SPM397): sc-56536 monoclonal antibody?
Preguntado por: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here:
Rated 5 de
Excellent!This antibody is recommended for western blot analysis of Gα i-1 expression in SK-N-SH whole cell lysate and rat brain and mouse brain tissue extracts.
Fecha de publicación: 2017-02-10
Rated 4 de
Cytoplasmic and membrane staining observedCytoplasmic and membrane staining observed by immunofluorescence of formalin fixed HepG2 cells. -SCBT QC
Fecha de publicación: 2015-05-07
Rated 5 de
Publishable data obtained by Western BlotPublishable data obtained by Western Blot using human A2780 and OVCAR3 cells. -SCBT Publication Review
Fecha de publicación: 2015-05-04
Rated 4 de
Light backgroundLight background, but crisp solid band observed by Western Blot using SK-N-SH whole cell lysate.
Fecha de publicación: 2015-01-22
Rated 4 de
Different expression levels of the G iDifferent expression levels of the G i-1 protein demonstrated by Western Blot analysis using SK-N-SH whole cell lysate, rat and mouse brain tissue extracts.
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