FAM63A (h): 293T Lysate: sc-114955

The FAM63A (h): 293T Lysate is derived from the HEK293T cell line, a human embryonic kidney cell line known for its high transfection efficiency and widespread use in research. This lysate contains FAM63A, a member of the MINDY (Motif Interacting with Ub-containing Novel DUB family) family of deubiquitinases. FAM63A specifically cleaves Lys-48-linked polyubiquitin chains, which are commonly associated with targeting proteins for proteasomal degradation. By removing these ubiquitin tags, FAM63A plays a crucial role in regulating protein stability and turnover, impacting various cellular processes including signal transduction, DNA repair, and cell cycle control. In research, the FAM63A (h): 293T Lysate is utilized to study the mechanisms of ubiquitin-mediated protein regulation. Researchers use this lysate to investigate the enzymatic activity of FAM63A, its substrate specificity, and its role in the ubiquitin-proteasome system. It is particularly valuable in in vitro assays to analyze protein-protein interactions, identify potential substrates, and understand the regulatory networks involving FAM63A. Additionally, this lysate aids in screening for modulators of FAM63A activity, providing insights into how alterations in deubiquitination can affect cellular functions and protein homeostasis. By leveraging this lysate, scientists gain a deeper understanding of the complex dynamics of ubiquitin signaling and the specific contributions of FAM63A to cellular regulation.