Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
EYA4 CRISPR/Cas9 KO Plasmid (h): sc-404355
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- EYA4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the EYA4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: EYA4 Antibody (E-11): sc-393111
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
EYA4 CRISPR/Cas9 KO Plasmid (h) | sc-404355 | 20 µg | $397.00 |
Overview
EYA4 encodes a member of the Eyes Absent (EYA) family that functions as a transcriptional coactivator and phosphatase, integrating developmental and stress-responsive signaling programs. Through its interactions with SIX and DACH proteins, EYA4 contributes to gene regulatory networks controlling cell fate decisions, differentiation, and tissue-specific morphogenesis. The protein’s tyrosine phosphatase activity has been linked to modulation of DNA damage responses and other phosphorylation-dependent processes that shape cellular homeostasis. Altered EYA4 expression or genomic disruption is associated with sensory and cardiac phenotypes and has also been reported in studies of tumor-associated epigenetic silencing, supporting its relevance for mechanistic investigations in human disease models.
EYA4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the EYA4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the EYA4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the EYA4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish EYA4 protein expression.
This CRISPR knockout system enables efficient generation of EYA4-deficient cell models for investigation of EYA4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting EYA4 exon(s) critical for EYA4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple EYA4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by EYA4 CRISPR/Cas9 KO Plasmid (h) and EYA4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the EYA4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by EYA4 HDR Plasmid (h) and EYA4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by EYA4 homology arms to support homology-directed repair at defined EYA4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.