Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Espin CRISPR/Cas9 KO Plasmid (h): sc-403106
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Espin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Espin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Espin Antibody (E-7): sc-393469
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Espin CRISPR/Cas9 KO Plasmid (h) | sc-403106 | 20 µg | $397.00 |
Overview
ESPN encodes espin, an actin-bundling protein that localizes to parallel actin filament bundles and is enriched in microvilli and stereocilia. Espin regulates cytoskeletal architecture, including protrusion elongation and stabilization, thereby shaping cell surface morphology and mechanosensory structures. Through modulation of actin dynamics and crosslinking, espin influences processes such as epithelial brush border organization and hair cell stereocilia maintenance. Altered ESPN function has been associated with sensory and cytoskeletal phenotypes relevant to hearing biology and actin-driven structural disorders.
Espin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ESPN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ESPN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ESPN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Espin protein expression.
This CRISPR knockout system enables efficient generation of ESPN-deficient cell models for investigation of Espin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ESPN exon(s) critical for Espin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ESPN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Espin CRISPR/Cas9 KO Plasmid (h) and Espin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ESPN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Espin HDR Plasmid (h) and Espin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ESPN homology arms to support homology-directed repair at defined ESPN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.