EphA4 Anticuerpo (D-4) recomendado para detectarEphA4 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA

EphA4 Anticuerpo (D-4): sc-365503

EphA4 Anticuerpo (D-4) clasificado 3.4 de 5 por 9.
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Ficha técnica

    • Anti-EphA4 Anticuerpo D-4 es un monoclonal de ratón IgG2b (kappa light chain) EphA4 Anticuerpo proporcionado como 200 µg/ml
    • específico para un epítopo localizado entre los amino ácidos 889-921 cerca del C-terminus de EphA4 oe orgiendf humanorigin
    • EphA4 Anticuerpo (D-4) es recomendado para detectar EphA4 de mouse, rat y human origen, mediante WB, IP, IF, IHC(P) y ELISA; también reactivo con otras especies, incluyendo anyd canine and porcine
    • EphA4 Anticuerpo (D-4) es disponible conjugado a agarosa para IP; HRP para WB, IHC(P) y ELISA; y tanto a phycoerythrin como a FITC para IF, IHC(P) y FCM
    • también disponible conjugado a Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 o Alexa Fluor® 647 para WB (RGB), IF, IHC (P) y FCM
    • también disponible conjugado a Alexa Fluor® 680 o Alexa Fluor® 790 para WB (NIR), IF y FCM
    • Contacte con nosotros para recibir GRATIS 10 µg de muestra de EphA4 (D-4): sc-365503.
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for EphA4 Antibody (D-4) for WB and IHC(P) applications. This reagent is now offered in a bundle with EphA4 Antibody (D-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What application is the blocking peptide sc-365503 P appropriate for?

Preguntado por: Randy McDonald
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Respondida por: Technical Support
Fecha de publicación: 2017-02-24
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Rated 5 de 5 por de Great antibody! This antibody works extremely well for WB on mouse cells.
Fecha de publicación: 2019-08-19
Rated 1 de 5 por de non-specific Test IF with this antibody on both embryonic and early postnatal mouse brain tissue. No specific signal could be detected.
Fecha de publicación: 2019-03-15
Rated 1 de 5 por de Not specific. Test IF with this antibody on both embryonic and early postnatal mouse brain tissue. No specific signal could be detected.
Fecha de publicación: 2019-03-15
Rated 4 de 5 por de Good immunoblots in human oral epithelial cells I tested this EphA4 antibody (free sample) in immuno blots. Our samples were human oral epithelial cells. The membrane was incubated with 1:1000 Ab in TBST-5% BSA at 4C for O/N. One band was detected at ~125 kDa. This antibody worked fine for us.
Fecha de publicación: 2017-07-25
Rated 1 de 5 por de This antibody does NOT work in our Western blot We tested this antibody (free sample from SC) in Western Blot (WB) multiple times. Unfortunately, it does NOT work. Our samples were from mouse neuronal stem cells, which have been confirmed containing EphA4 in both IHC and WB by using other Abs. In our experiment, each lane was loaded by 100 ug total protein. The membrane was incubated with 1:1000 Ab in TBST-5% BSA at 4C for O/N. According to our results, there were multiple bands > 150 kDa , but no band at 110-120 kDa which is the expected Mw of EphA4.
Fecha de publicación: 2016-12-20
Rated 4 de 5 por de Potent and fairly specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~120 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, except for three major cross-reactive proteins of ~32, 48 and 62 kDa were also detected.
Fecha de publicación: 2016-11-10
Rated 5 de 5 por de Good publishable data in IF in chicken retina Good publishable data in IF in chicken retina tissue. -SCBT Publication Review
Fecha de publicación: 2015-04-25
Rated 5 de 5 por de Produced excellent immunofluorescence membrane Produced excellent immunofluorescence membrane staining in methanol-fixed HeLa cells. -SCBT QC
Fecha de publicación: 2015-03-05
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