desmoplakin I/II CRISPR/Cas9 KO Plasmid (m): sc-430943
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- desmoplakin I/II CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the desmoplakin I/II genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
desmoplakin I/II CRISPR/Cas9 KO Plasmid (m) | sc-430943 | 20 µg | $397.00 |
Overview
Mouse Dsp encodes desmoplakin I/II, a core cytolinker of desmosomes that anchors intermediate filaments to desmosomal cadherin–plakoglobin/plakophilin complexes, supporting tissue integrity under mechanical stress. Desmoplakin regulates cell–cell adhesion, cytoskeletal organization, and junctional signaling networks that coordinate epithelial and cardiac muscle architecture. Disruption of desmosomal function alters mechanotransduction and barrier properties, affecting processes such as epidermal differentiation, wound response, and cardiomyocyte coupling. Dsp dysfunction is widely used as a model for desmosome-related pathobiology, including skin fragility phenotypes and cardiomyopathy-associated junctional defects.
desmoplakin I/II CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Dsp gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Dsp together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Dsp open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish desmoplakin I/II protein expression.
This CRISPR knockout system enables efficient generation of Dsp-deficient cell models for investigation of desmoplakin I/II signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Dsp exon(s) critical for desmoplakin I/II function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Dsp genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by desmoplakin I/II CRISPR/Cas9 KO Plasmid (m) and desmoplakin I/II CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Dsp locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by desmoplakin I/II HDR Plasmid (m) and desmoplakin I/II HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Dsp homology arms to support homology-directed repair at defined Dsp target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.