DDX17 Antibody (H-7): sc-398168

DDX17 Antibody (H-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects DDX17 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-DDX17 antibody (H-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. DDX17, also known as DEAD box protein 17 or p72, plays a crucial role in RNA metabolism, particularly in unwinding RNA structures, which is essential for processes such as translation initiation and splicing. This unwinding activity is vital for proper functioning of ribosomes and spliceosomes, which are necessary for protein synthesis and gene expression regulation. DDX17′s involvement in these fundamental cellular processes highlights its importance in maintaining cellular homeostasis and regulating developmental pathways. DDX17 has been shown to interact with several transcription factors, including estrogen receptor alpha, p53, MyoD, and Runx2, thereby influencing growth regulation and differentiation. Dysregulation of DDX17 has been linked to developmental disorders such as Down Syndrome and has been implicated in colon cancer progression, making DDX17 a potential therapeutic target for cancer treatment.

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DDX17 Antibody (H-7) References:

1. The mRNA of DEAD box protein p72 is alternatively translated into an 82-kDa RNA helicase.  |  Uhlmann-Schiffler, H., et al. 2002. J Biol Chem. 277: 1066-75. PMID: 11675387
2. Reduced levels of DEAD-box proteins DBP-RB and p72 in fetal Down syndrome brains.  |  Kircher, SG., et al. 2002. Neurochem Res. 27: 1141-6. PMID: 12462412
3. The p68 and p72 DEAD box RNA helicases interact with HDAC1 and repress transcription in a promoter-specific manner.  |  Wilson, BJ., et al. 2004. BMC Mol Biol. 5: 11. PMID: 15298701
4. RNA helicases: regulators of differentiation.  |  Abdelhaleem, M. 2005. Clin Biochem. 38: 499-503. PMID: 15885226
5. Redundant role of DEAD box proteins p68 (Ddx5) and p72/p82 (Ddx17) in ribosome biogenesis and cell proliferation.  |  Jalal, C., et al. 2007. Nucleic Acids Res. 35: 3590-601. PMID: 17485482
6. Involvement of RNA helicases p68 and p72 in colon cancer.  |  Shin, S., et al. 2007. Cancer Res. 67: 7572-8. PMID: 17699760
7. The DEAD box RNA helicases p68 (Ddx5) and p72 (Ddx17): novel transcriptional co-regulators.  |  Fuller-Pace, FV. and Ali, S. 2008. Biochem Soc Trans. 36: 609-12. PMID: 18631126
8. Dual role of the ddx5/ddx17 RNA helicases in the control of the pro-migratory NFAT5 transcription factor.  |  Germann, S., et al. 2012. Oncogene. 31: 4536-49. PMID: 22266867
9. The Ddx5 and Ddx17 RNA helicases are cornerstones in the complex regulatory array of steroid hormone-signaling pathways.  |  Samaan, S., et al. 2014. Nucleic Acids Res. 42: 2197-207. PMID: 24275493
10. The oncogenic JAG1 intracellular domain is a transcriptional cofactor that acts in concert with DDX17/SMAD3/TGIF2.  |  Kim, EJ., et al. 2022. Cell Rep. 41: 111626. PMID: 36417870
11. LncRNA FAM225B Regulates PDIA4-Mediated Ovarian Cancer Cell Invasion and Migration via Modulating Transcription Factor DDX17.  |  Yao, C., et al. 2023. Breast J. 2023: 3970444. PMID: 37720188
12. p72: a human nuclear DEAD box protein highly related to p68.  |  Lamm, GM., et al. 1996. Nucleic Acids Res. 24: 3739-47. PMID: 8871553

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-DDX17 Antibody (H-7) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-DDX17 Antibody (H-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

DDX17 Antibody (H-7) Specifications

Alternate Product Name: DDX17

Product Clone Name: H-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 143-155

Epitope Target: DDX17

Epitope Species: human

Product Reactivity/Detection: DDX17

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for DDX17 Antibody (H-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin