Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
DBP Double Nickase Plasmid (h): sc-403069-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DBP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DBP Double Nickase Plasmid (h) and DBP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GC. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: DBP Antibody (A-5): sc-365441
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DBP Double Nickase Plasmid (h) | sc-403069-NIC | 20 µg | $410.00 | |||
DBP Double Nickase Plasmid (h2) | sc-403069-NIC-2 | 20 µg | $410.00 |
Human GC encodes vitamin D–binding protein (DBP), a highly abundant plasma glycoprotein that binds and transports vitamin D metabolites and contributes to systemic regulation of calcium and phosphate homeostasis. Beyond vitamin D carriage, DBP participates in actin scavenging and can influence immune and inflammatory processes through modulation of extracellular actin clearance and leukocyte responses. Variation in GC/DBP levels and binding properties has been studied in the context of bone and mineral disorders, metabolic phenotypes, and inflammatory conditions. In research settings, GC provides a tractable node for probing vitamin D signaling dynamics, endocrine crosstalk, and extracellular protein handling.
DBP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GC locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GC. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GC function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GC-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.