COPE Antibody (A-4): sc-133195

COPE Antibody (A-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects COPE protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-COPE antibody (A-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. COPE protein plays a crucial role in membrane and vesicular trafficking within the early secretory pathway, specifically mediating retrograde transport from the Golgi apparatus back to the endoplasmic reticulum, as well as intra-Golgi transport through non-Clathrin coat protein I-coated vesicles. The heptameric coatomer complex, which is the cytosolic precursor of the COPI coat, consists of two subcomplexes: the first includes the β-, γ-, δ-, and ζ-COP subunits, while the second comprises the α-COP, COPP, and ε-COP subunits. Understanding the structure and function of COPE protein is vital, as COPE protein is essential for maintaining cellular homeostasis and ensuring proper protein sorting and trafficking, which are critical for various cellular processes and overall cell function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

COPE Antibody (A-4) References:

1. Protein targeting to endoplasmic reticulum by dilysine signals involves direct retention in addition to retrieval.  |  Andersson, H., et al. 1999. J Biol Chem. 274: 15080-4. PMID: 10329713
2. The epithelial Na(+)/H(+) exchanger, NHE3, is internalized through a clathrin-mediated pathway.  |  Chow, CW., et al. 1999. J Biol Chem. 274: 37551-8. PMID: 10608808
3. Impaired proteasome function rescues thermosensitivity of yeast cells lacking the coatomer subunit epsilon-COP.  |  Kimata, Y., et al. 2000. J Biol Chem. 275: 10655-60. PMID: 10744762
4. In vitro assembly and disassembly of coatomer.  |  Lowe, M. and Kreis, TE. 1995. J Biol Chem. 270: 31364-71. PMID: 8537409
5. Inhibition of endosome function in CHO cells bearing a temperature-sensitive defect in the coatomer (COPI) component epsilon-COP.  |  Daro, E., et al. 1997. J Cell Biol. 139: 1747-59. PMID: 9412469
6. epsilon-COP is a structural component of coatomer that functions to stabilize alpha-COP.  |  Duden, R., et al. 1998. EMBO J. 17: 985-95. PMID: 9463377
7. A single binding site for dilysine retrieval motifs and p23 within the gamma subunit of coatomer.  |  Harter, C. and Wieland, FT. 1998. Proc Natl Acad Sci U S A. 95: 11649-54. PMID: 9751720

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-COPE Antibody (A-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-COPE Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

COPE Antibody (A-4) Specifications

Alternate Product Name: COPE

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 111-190

Epitope Target: COPE

Epitope Species: human

Product Reactivity/Detection: COPE

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for COPE Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin