Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
COL8A2 Double Nickase Plasmid (h): sc-403831-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL8A2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COL8A2 Double Nickase Plasmid (h) and COL8A2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COL8A2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COL8A2 Antibody (1F4): sc-293350
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL8A2 Double Nickase Plasmid (h) | sc-403831-NIC | 20 µg | $410.00 | |||
COL8A2 Double Nickase Plasmid (h2) | sc-403831-NIC-2 | 20 µg | $410.00 |
COL8A2 encodes the α2 chain of type VIII collagen, a short-chain non-fibrillar collagen that contributes to specialized basement membrane and extracellular matrix (ECM) assemblies. It is expressed prominently in ocular tissues, where it supports corneal endothelial and Descemet’s membrane structure and modulates cell–matrix interactions that influence adhesion, migration, and differentiation. COL8A2-linked ECM remodeling intersects with integrin-mediated signaling, focal adhesion dynamics, and TGF-β–responsive matrix programs that shape tissue biomechanics and cellular stress responses. Genetic variation and dysregulated expression of COL8A2 are associated with corneal endothelial dystrophies, making it a relevant target for studying ECM homeostasis, proteostasis, and endothelial cell biology in human models.
COL8A2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COL8A2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COL8A2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COL8A2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COL8A2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.