COL8A1 CRISPR/Cas9 KO Plasmid (m): sc-419753
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COL8A1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the COL8A1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COL8A1 CRISPR/Cas9 KO Plasmid (m) | sc-419753 | 20 µg | $397.00 |
Overview
Col8a1 encodes collagen type VIII alpha 1 (COL8A1), a non-fibrillar extracellular matrix collagen enriched in basement membrane–associated matrices and vascular-associated connective tissues. COL8A1 contributes to matrix assembly, cell–matrix adhesion, and regulation of cellular behaviors such as migration and proliferation through interactions with integrins and remodeling networks involving MMP activity. In mouse systems, altered Col8a1 expression and collagen VIII deposition are frequently studied in contexts of vascular remodeling, fibrotic matrix accumulation, and wound-associated extracellular matrix reorganization. These processes intersect with TGF-β–responsive programs and broader extracellular matrix pathways that shape tissue architecture and inflammatory microenvironments.
COL8A1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Col8a1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Col8a1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Col8a1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish COL8A1 protein expression.
This CRISPR knockout system enables efficient generation of Col8a1-deficient cell models for investigation of COL8A1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Col8a1 exon(s) critical for COL8A1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Col8a1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by COL8A1 CRISPR/Cas9 KO Plasmid (m) and COL8A1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Col8a1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by COL8A1 HDR Plasmid (m) and COL8A1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Col8a1 homology arms to support homology-directed repair at defined Col8a1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.