CDKL5 Double Nickase Plasmid (h): sc-403409-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CDKL5 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CDKL5 Double Nickase Plasmid (h) and CDKL5 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDKL5. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CDKL5 Antibody (D-12): sc-376314
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDKL5 Double Nickase Plasmid (h) | sc-403409-NIC | 20 µg | $410.00 | |||
CDKL5 Double Nickase Plasmid (h2) | sc-403409-NIC-2 | 20 µg | $410.00 |
CDKL5 encodes cyclin-dependent kinase-like 5, a serine/threonine kinase that is highly expressed in the nervous system and supports neuronal maturation, synaptic function, and activity-dependent signaling. CDKL5 regulates phosphorylation-dependent processes that influence chromatin dynamics, gene expression programs, and cytoskeletal organization, linking it to pathways governing neurite outgrowth and synaptic plasticity. Disruption or reduced function of CDKL5 is associated with severe neurodevelopmental phenotypes, making it a key target for mechanistic studies of kinase signaling in neurons. In cell models, CDKL5 perturbation is used to investigate how altered phosphorylation cascades impact neuronal network development and stimulus-responsive transcription.
CDKL5 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDKL5 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDKL5. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDKL5 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDKL5-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.