CDK2AP2 CRISPR/Cas9 KO Plasmid (h): sc-406842
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CDK2AP2 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CDK2AP2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDK2AP2 CRISPR/Cas9 KO Plasmid (h) | sc-406842 | 20 µg | $397.00 |
Overview
CDK2AP2 (CDK2-associated protein 2) is a cell cycle–linked regulator implicated in controlling CDK2 activity and coordinating G1/S transition with DNA replication programs. The protein is associated with nuclear processes that influence proliferation, chromatin dynamics, and maintenance of genome integrity, making it relevant to studies of replicative stress and checkpoint control. Altered regulation of CDK2AP2-dependent pathways has been explored in the context of dysregulated cell growth and transformation-associated phenotypes, supporting its use as a molecular handle for interrogating cell cycle circuitry. In human cell models, CDK2AP2 perturbation can help define how CDK2-centered networks interface with transcriptional and DNA damage response modules.
CDK2AP2 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDK2AP2 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDK2AP2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDK2AP2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CDK2AP2 protein expression.
This CRISPR knockout system enables efficient generation of CDK2AP2-deficient cell models for investigation of CDK2AP2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDK2AP2 exon(s) critical for CDK2AP2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDK2AP2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CDK2AP2 CRISPR/Cas9 KO Plasmid (h) and CDK2AP2 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDK2AP2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CDK2AP2 HDR Plasmid (h) and CDK2AP2 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDK2AP2 homology arms to support homology-directed repair at defined CDK2AP2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.